Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification.

The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.