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TF Activation Plate Arrays

Signosis’ TF Activation Profiling Plate Array enables the simultaneous profiling of up to 192 transcription factors in a single experiment. This high-throughput assay streamlines workflow, reduces cost, and provides a comprehensive snapshot of transcriptional activity, supporting research in cancer, metabolic disorders, and neurodegeneration.

Principle 

Signosis’ TF Activation Profiling Arrays are designed to monitor the activity of multiple transcription factors (TFs) simultaneously. In this assay, biotin-labeled probes, based on consensus sequences of TF DNA-binding sites, are incubated with nuclear extracts. When a transcription factor is activated, it binds to its corresponding probe, forming a TF/probe complex. This complex can then be separated from unbound probes through a simple spin-column purification.
 

After the binding reaction, the complexes are denatured, and the biotin-labeled probes are released and hybridized to complementary sequences pre-coated on the detection plate. This process allows for the high-throughput detection of transcription factor activity.
 

The TF Activation Plates come in three categories:

  • Few Cell TF: Designed for screening with a limited number of cells.

  • TF Activation Plate: Enables the screening of 48 to 192 transcription factors.

  • Functional TF: Focuses on transcription factors involved in specific pathways, such as ER Stress, Cholesterol metabolism, & much more.

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Product Categories

TF Profiling Plate Array

Enables the screening of 48 to 192 transcription factors.

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FewCell TF Plate Arrays

Designed for screening with a limited number of cells.

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Functional Plate Arrays

Focuses on transcription factors involved in specific pathways.

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Specifications

TF Profiling Plate Arrays

  HIGH THROUGHPUT

Can profile up to 192 transcription factors (TFs) binding to a promoter of interest in a single experiment. 

SAMPLE TYPE

requires nuclear extract as the starting material, which can either be purchased with the kit or separately. The nuclear extract, catalog number SK-0001, is suitable for both mammalian tissue and cultured cells. 

# OF SAMPLES

Able to run up to 2 samples.

CUSTOMIZATION

We offer the flexibility to customize the plate or synthesize a specific transcription factor if the one you need is not available in our standard kit. Let us know your requirements, and we can tailor the assay to suit your research needs. 

***Notes: Each product has its own tailored manual and plate layout, designed for the specific transcription factors and assay features. To view the manual and plate layout for the product you're interested in, click on the product page. You’ll find the manual and plate layout under the "Tabs" section.

Benefits

High-Throughput Efficiency

Analyze multiple TFs in parallel, reducing experimental time by up to 90%

​Cost-Effective

​Reduces the need for extensive sequencing or individual TF assays, lowering costs while increasing data output.

Enhanced Sensitivity

Detect subtle changes in TF activation, crucial for understanding complex signaling networks.

​Works with Mammalian Nuclear Extracts

Samples can be taken from human, mouse, or rat and more.

Streamlined Workflow

​No specialized molecular biology expertise is required - simple prepare nuclear extracts with our Nuclear Extraction Kit and follow the straightforward protocol

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