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TF Activation Plate Arrays

Signosis’ TF Activation Profiling Plate Array enables the simultaneous profiling of up to 192 transcription factors in a single experiment. This high-throughput assay streamlines workflow, reduces cost, and provides a comprehensive snapshot of transcriptional activity, supporting research in cancer, metabolic disorders, and neurodegeneration.

Principle 

Signosis’ TF Activation Profiling Arrays are designed to monitor the activity of multiple transcription factors (TFs) simultaneously. In this assay, biotin-labeled probes, based on consensus sequences of TF DNA-binding sites, are incubated with nuclear extracts. When a transcription factor is activated, it binds to its corresponding probe, forming a TF/probe complex. This complex can then be separated from unbound probes through a simple spin-column purification.
 

After the binding reaction, the complexes are denatured, and the biotin-labeled probes are released and hybridized to complementary sequences pre-coated on the detection plate. This process allows for the high-throughput detection of transcription factor activity.
 

The TF Activation Plates come in three categories:

  • Few Cell TF: Designed for screening with a limited number of cells.

  • TF Activation Plate: Enables the screening of 48 to 192 transcription factors.

  • Functional TF: Focuses on transcription factors involved in specific pathways, such as ER Stress, Cholesterol metabolism, & much more.

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Product Categories

TF Profiling Plate Array

Enables the screening of 48 to 192 transcription factors.

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FewCell TF Plate Arrays

Designed for screening with a limited number of cells.

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Functional Plate Arrays

Focuses on transcription factors involved in specific pathways.

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Specifications

TF Profiling Plate Arrays

  HIGH THROUGHPUT

Can profile up to 192 transcription factors (TFs) binding to a promoter of interest in a single experiment. 

SAMPLE TYPE

requires nuclear extract as the starting material, which can either be purchased with the kit or separately. The nuclear extract, catalog number SK-0001, is suitable for both mammalian tissue and cultured cells. 

# OF SAMPLES

Able to run up to 2 samples.

CUSTOMIZATION

We offer the flexibility to customize the plate or synthesize a specific transcription factor if the one you need is not available in our standard kit. Let us know your requirements, and we can tailor the assay to suit your research needs. 

***Notes: Each product has its own tailored manual and plate layout, designed for the specific transcription factors and assay features. To view the manual and plate layout for the product you're interested in, click on the product page. You’ll find the manual and plate layout under the "Tabs" section.

Benefits

Broad Pathway Coverage

Profiles nearly all major signaling pathways (e.g., NFkB, STATs, p53) in one assay—ideal for capturing global transcriptional activity.

Mechanism-of-Action & Pathway Profiling

Quickly reveals pathway activation and interactions in response to drugs or genetic modifications.

Works with Mammalian Nuclear Extracts

Samples can be taken from all mammalian cells including human, mouse, or rat and more.

Discovery-Driven Research

Perfect for exploring unknown regulatory mechanisms in novel treatments or diseases.

Biomarker Discovery & Validation

Uncovers TF signatures linked to disease, treatment response, or prognosis—widely used in cancer and immunology.

Saves Time, Sample & Cost

Consolidates multiple assays into one—especially useful for limited or precious samples.

High-Throughput Ready

Compatible with automation and large-scale applications like drug screening or CRISPR studies.

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