In Signosis' Transcription Factor (TF) Filter Plate Assays, a biotin-labeled DNA probe containing a specific TF binding sequence is mixed with nuclear extracts to form TF-DNA complexes. These complexes are captured on a filter plate, which retains the bound probe while washing away any unbound probe. The bound probe is then eluted from the filter and transferred to a DNA hybridization plate for quantification. Detection is performed using streptavidin-HRP, and the resulting luminescence is measured as relative light units (RLUs) using a microplate luminometer.