Cytokine ELISA Plate Arrays
Profile cytokine expression in cells, serum, or other biological samples using Signosis’ ELISA Plate Array Kits, designed to simultaneously detect 32 or more cytokines in a user-friendly 96-well format. This multiplex format saves time and minimizes sample usage, while enabling broad and reliable cytokine profiling in one easy-to-use assay.
Principle
In this assay, cytokines in the sample bind to specific capture antibodies pre-coated on a 96-well plate. After washing away unbound materials, a biotin-labeled detection antibody mix targeting all included cytokines is added to form cytokine-antibody complexes. Streptavidin-HRP is then introduced to bind the biotin-labeled antibodies, completing the detection complex.
Two detection formats are available:
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Colorimetric kits: TMB substrate is added, producing a blue color in the presence of HRP. The reaction is stopped with Stop Solution, turning the mixture yellow. Absorbance is measured at 450 nm, and the signal intensity is directly proportional to the cytokine concentration.
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Chemiluminescent kits: A luminescent HRP substrate is added, and the resulting signal is measured as relative light units (RLUs) using a plate luminometer. The light intensity corresponds to the amount of cytokine present in the sample.
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Benefits
Easy-to-use & Highly Sensitive
Simple procedure with ready to use reagents and requires minimal amount of sample. Compatible with common plate readers.
Multiplex
One pre-coated plate can measure 48 cytokines for 2 human samples, 32 cytokines for 3 human samples; 32 cytokines for 3 mouse samples, 24 cytokines for 4 mouse samples; or 16 cytokines for 6 rat samples.
Cytokine Profiling
Can accurately compare cytokine levels between samples.
Compatibility
The assay is suitable for any biological liquid material such as cell lysate, sera, cerebrospinal fluid (CSF), urine, and tissue homogenate.
