Signosis' Oxidative Stress Combo Assay Kits are multiplex assays designed to simultaneously measure key biomarkers of oxidative stress and antioxidant activity: ROS, MDA, GSH, SOD, GPx, and catalase. Each marker is detected using a distinct and validated method:

• Reactive Oxygen Species (ROS) are detected via a fluorescence-based probe that emits signal in response oxidation.

• Malondialdehyde (MDA), a lipid peroxidation byproduct, is quantified using a colorimetric reaction with thiobarbituric acid (TBA).

• Glutathione (GSH) reacts with DTNB to form a yellow-colored product, and the color intensity reflects the amount of GSH in the sample.

• Superoxide Dismutase (SOD) activity is determined by measuring its ability to inhibit the reduction of a tetrazolium salt by superoxide radicals.

• Glutathione Peroxidase (GPx) activity is determined by measuring its ability to reduce hydrogen peroxide in the presence of glutathione.

• Catalase activity is determined by measuring its ability to decompose hydrogen peroxide.

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