Oxidative Stress/Antioxidant
Assay Kits
Signosis’ Oxidative Stress Combo Assay Kits provide a comprehensive solution for analyzing multiple oxidative stress and antioxidant markers in a variety of sample types, including cells, tissues, blood, and other biological fluids. This innovative multiplex assay kit simultaneously measures key oxidative stress and antioxidant markers: reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase, and provides researchers with an essential tool for studying oxidative stress in a variety of diseases, such as cancer, neurodegenerative diseases, and cardiovascular disorders. The Oxidative Damage ELISA Combo Kit can be used in conjunction with the Oxidative Stress Assay Kits to measure biomarkers of long term oxidative damage.
How to measure Oxidative Stress?
Oxidative stress can be assessed in several ways. It can be measured directly by analyzing the production of reactive oxygen species (ROS) using the fluorescent probe DCFDA. Alternatively, oxidative stress can be inferred by evaluating lipid peroxidation by measuring MDA, which reflects damage to cell membranes caused by ROS. Additionally, the body’s antioxidant defense system can be assessed, including key enzymes and molecules such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Together, these approaches provide a comprehensive picture of oxidative stress and the body’s capacity to counteract it.
Principle
Signosis' Oxidative Stress Combo Assay Kits are multiplex assays designed to simultaneously measure key biomarkers of oxidative stress and antioxidant activity: ROS, MDA, GSH, SOD, GPx, and catalase. Each marker is detected using a distinct and validated method:
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Reactive Oxygen Species (ROS) are detected via a fluorescence-based probe that emits signal in response oxidation.
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Malondialdehyde (MDA), a lipid peroxidation byproduct, is quantified using a colorimetric reaction with thiobarbituric acid (TBA).
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Glutathione (GSH) reacts with DTNB to form a yellow-colored product, and the color intensity reflects the amount of GSH in the sample.
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Superoxide Dismutase (SOD) activity is determined by measuring its ability to inhibit the reduction of a tetrazolium salt by superoxide radicals.
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Glutathione Peroxidase (GPx) activity is determined by measuring its ability to reduce hydrogen peroxide in the presence of glutathione.
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Catalase activity is determined by measuring its ability to decompose hydrogen peroxide.
Products
Benefits
Streamlined Workflow
Includes all necessary reagents for simple and efficient analysis.
Multiplex Capability
Measure multiple oxidative stress and antioxidant markers simultaneously from the same sample.
Compatible with Multiple Sample Types
Specialized protocols are designed to analyze cells, tissue, or blood/biological fluid samples.
High Sensitivity
Accurately detect low-abundance analytes for precise measurements.