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Oxidative Stress/Antioxidant
Assay Kits

Signosis’ Oxidative Stress Combo Assay Kits provide a comprehensive solution for analyzing multiple oxidative stress markers in a variety of sample types, including cells, tissues, blood, and other biological fluids. This innovative multiplex assay kit simultaneously measures four key oxidative stress and antioxidant markers: reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD), and provides researchers with an essential tool for studying oxidative stress in a variety of diseases, such as cancer, neurodegenerative diseases, and cardiovascular disorders.

Principle 

The Signosis Oxidative Stress Combo Assay Kit is a multiplex assay designed to simultaneously measure four key biomarkers of oxidative stress: ROS, MDA, GSH, and SOD. Each marker is detected using a distinct and validated method:

  • Reactive Oxygen Species (ROS) are detected via a fluorescence-based probe that emits signal upon oxidation.

  • Malondialdehyde (MDA), a lipid peroxidation byproduct, is quantified using a colorimetric reaction with thiobarbituric acid (TBA).

  • Glutathione (GSH) reacts with DTNB to form a yellow-colored product, and the color intensity reflects the amount of GSH in the sample.

  • Superoxide Dismutase (SOD) activity is determined by its ability to inhibit the reduction of a tetrazolium salt by superoxide radicals.

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Benefits

Streamlined Workflow

Includes all necessary reagents for simple and efficient analysis.

Multiplex Capability

​Measure multiple oxidative stress and antioxidant markers simultaneously from the same sample.

Compatible with Multiple Sample Types

Specialized protocols are designed to analyze cells, tissue, or blood/biological fluid samples.

High Sensitivity

Accurately detect low-abundance analytes for precise measurements.

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