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Oxidative Stress Detection Assay Kits

Oxidative stress is caused by an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to detoxify them (antioxidant) or repair the resulting damage. ROS are highly reactive molecules containing oxygen, such as superoxide anion, hydroxyl radical, and hydrogen peroxide H2O2. When the production of ROS exceeds the capacity of antioxidant defenses, oxidative stress occurs and leads to cell and tissue damage. Signosis has developed and launched the simple and effective Oxidative Stress Detection Assay Kits, Amplex Red H2O2 Assay Kit to detect extracellular H2O2, and DCFDA ROS Assay Kit to detect intracellular total ROS.

Principle

Amplex Red H2O2 Assay


The Hydrogen Peroxide (H2O2) Assay kit utilizes the Amplex Red reagent and horseradish peroxidase (HRP) to detect H2O2 in biological samples, such as cell media. Because cells secrete H2O2 into their surrounding environment, cells under oxidative stress can be analyzed by measuring their extracellular H2O2 levels. The H2O2 levels in samples is quantified with the Amplex Red reagent, which reacts with HRP and H2O2 to produce a red fluorescent signal.

DCFDA ROS Assay


The DCFDA (H2DCFDA) Assay kit utilizes 2’,7’ –dichlorofluorescin diacetate (DCFDA), a cell permeable reagent, to detect reactive oxygen species in live cells. DCFDA is capable of measuring hydroxyl, peroxyl, and other reactive oxygen species activity within the cell. When DCFDA enters the cell, it is deacetylated by cellular esterases into a non-fluorescent compound. Then, it is oxidized by reactive oxygen species into 2’, 7’ –dichlorofluorescein (DCF), which is highly fluorescent and can be detected by fluorescence spectroscopy.
 

Benefits

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