Oxidative Stress/Antioxidant
Assay Kits
Signosis' Oxidative Stress Combo Assay Kits provide a comprehensive solution for analyzing multiple oxidative stress and antioxidant markers in a variety of sample types, including cells, tissues, blood, and other biological fluids. These innovative multiplex assay kits simultaneously measure key markers including reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD), providing researchers with a convenient and efficient solution for evaluating oxidative stress and antioxidant status across a wide range of disease models and biological samples.
The Oxidative Damage ELISA Combo Kit can be used alongside the Oxidative Stress Combo Assay Kits to evaluate biomarkers of long-term oxidative damage. In addition, Signosis offers standalone assay kits for glutathione peroxidase (GPx) and catalase, enabling researchers to further expand their oxidative stress analysis workflow.
How is Oxidative Stress Measured?
Oxidative stress can be assessed in several ways. Direct measurement is commonly performed by analyzing the production of reactive oxygen species (ROS) using the fluorescent probe DCFDA. Oxidative stress can also be evaluated by measuring malondialdehyde (MDA), a marker of lipid peroxidation that reflects ROS-induced damage to cell membranes. In addition, antioxidant defense capacity can be assessed through key enzymes and molecules such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Together, these measurements provide a comprehensive assessment of oxidative stress and antioxidant defense capacity.
Principle
Signosis' Oxidative Stress Combo Assay Kits are multiplex assays designed to simultaneously measure key biomarkers of oxidative stress and antioxidant activity, including ROS, MDA, GSH, and SOD. Each marker is detected using a distinct and validated method:
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Reactive Oxygen Species (ROS) are detected using a fluorescence-based probe that generates a fluorescent signal in response to oxidation.
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Malondialdehyde (MDA), a lipid peroxidation byproduct, is quantified using a colorimetric reaction with thiobarbituric acid (TBA).
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Glutathione (GSH) reacts with DTNB to form a yellow-colored product, and the color intensity reflects the amount of GSH in the sample.
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Superoxide Dismutase (SOD) activity is determined by measuring its ability to inhibit the reduction of a tetrazolium salt by superoxide radicals.
Additional standalone assay kits are available for measuring glutathione peroxidase (GPx) and catalase activity:
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Glutathione Peroxidase (GPx) activity is determined by measuring its ability to reduce hydrogen peroxide in the presence of glutathione.
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Catalase activity is determined by measuring its ability to decompose hydrogen peroxide.
Products
Benefits
Streamlined Workflow
Includes all necessary reagents for simple and efficient analysis.
Multiplex Capability
Measure multiple oxidative stress and antioxidant markers simultaneously from the same sample.
Compatible with Multiple Sample Types
Specialized protocols are designed to analyze cells, tissue, or blood/biological fluid samples.
High Sensitivity
Accurately detect low-abundance analytes for precise measurements.