Luciferase Substrate
Detect Firefly, Renilla, and Gaussia luciferases in a single, ready-to-use solution — no separate reagents or multi-step prep. Compatible with our lysis buffer and reliable with all reporter vectors and stable cell lines. Perfect for transcription factor assays, gene expression studies, cellular pathway analysis, and high-throughput screening. Ships on blue ice at 4°C — no dry ice needed — making experiments fast, simple, and cost-effective.
Principle
Our Firefly luciferase substrate is based on D-luciferin and produces a stable glow-type signal, ideal for high-throughput screening applications. The Gaussia luciferase substrate, using coelenterazine, generates an exceptionally bright flash-type signal, with greater intensity than Firefly or Renilla luciferase. These substrates are extensively validated with our Luciferase Reporter Stable Cell Lines, reporter vectors, and other commercial systems utilizing these luciferases, ensuring consistent and reliable performance. They are formulated for outstanding stability—maintaining performance for at least 48 hours at room temperature and a minimum of 4 weeks at 4°C. Their one-solution format simplifies workflows, reduces variability, and enhances reproducibility across luciferase-based assays.
Products
Product Name | SKU | Price | # of Reactions |
|---|---|---|---|
Firefly Luciferase Kit: Lysis (10 ml) & Substrate (100 ml) Bundle | $815.00 | ||
Firefly Luciferase Kit: Lysis (10 ml) & Substrate (15 ml) Bundle | $315.00 | ||
Firefly Luciferase Substrate (100mL) | $767.00 | 2000 | |
Firefly Luciferase Substrate (15mL) | $231.00 | 300 | |
Firefly Luciferase Lysis Buffer 5X (10mL) | $96.00 | 200 |
Product Name | SKU | Price | # of Reactions |
|---|---|---|---|
Renilla Luciferase Kit: Lysis (15 mL) & Substrate (50 mL) Bundle | $780.00 | ||
Renilla Luciferase Kit: Lysis (15 mL) & Substrate (10 mL) Bundle | $316.00 | ||
Renilla Luciferase Substrate (10mL) | $220.00 | 200 | |
Renilla Luciferase Substrate (50mL) | $700.00 | 1000 | |
Gaussia Luciferase Substrate (10mL) | $220.00 | 200 | |
Gaussia Luciferase Substrate (50mL) | $700.00 | 1000 |
Benefits
Ships Easily on Blue Ice — No Dry Ice Needed
Ships on blue ice at 4°C, saving you the hassle and cost of dry ice — so your experiments start faster and easier.
Compatibility
Works with all Signosis luciferase products as well as other third-party firefly luciferase systems.
High Sensitivity and Responsiveness
Produces a strong and stable light signal.
Cost-Effective
Lower cost per reaction compared to our competitors.
Simple, Easy-to-use, One-Step Solution
Ready to use without any prior preparation or mixing.