Luciferase Substrates
Detect Firefly, Renilla, and Gaussia luciferases in a single, ready-to-use solution with no preparation required. Compatible with our lysis buffer and all reporter vectors and stable cell lines. Ideal for transcription factor assays, gene expression studies, cellular pathway analysis, and high-throughput screening. Ships on blue ice at 4°C—no dry ice needed—making experiments fast, simple, and cost-effective.
Principle
We offer highly sensitive, ready-to-use substrates for Firefly, Renilla, and Gaussia luciferase detection in a convenient one-solution format. Our Firefly substrates have been extensively validated with our Luciferase Reporter Stable Cell Lines, reporter vectors, and other commercial used luciferase systems. Our Dual Luciferase Assay Systems support assays using our 2 in 1: Firefly–Renilla Reporter Vectors where Renilla serves as a normalization control, while our Gaussia substrate is designed for use with secreted Gaussia Luciferase Reporter Stable Cell Lines, enabling sensitive, real-time detection without lysing cells.
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Superior Stability: Retains efficacy when stored at room temperature for at least 48 hours and at 4°C for a minimum of 4 weeks, allowing shipment without expensive dry ice.
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Strong, Consistent Signal: Firefly Luciferase substrate, based on D-luciferin, provides a robust and stable glow ideal for high-throughput applications.
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One-Solution Convenience: No resuspension required; ready to use straight from the bottle.
By combining long-term stability, strong signal, and a simple one-solution format, our substrates help streamline workflows, minimize variability, and improve reproducibility. This makes them an affordable, reliable, and efficient solution for any luciferase-based assay.
Products
Benefits
4°C Shipping
Ships on blue ice at 4°C.
No expensive dry ice required.
High Sensitivity and Responsiveness
Produces a strong and stable light signal.
Best Value
Lower cost per reaction compared to our competitors due to optimized reagent use.
Validated Performance
Works with all Signosis luciferase products as well as other third-party firefly luciferase systems.
Ready to Use
Convenient one-step solution with no mixing or prep required.