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Luciferase Reporter Cell Lines for Monitoring
TF Binding and Activation
Gene function analysis, target discovery and validation, assay development, and compound screening often need cell-based assays. Stable cell lines that are engineered to express a gene of interest via transgene integrations into the host genome provide an efficient approach to conduct such analysis.
At Signosis, we help researchers save time and labor in generating and validating stable cell lines so scientists can spend more effort on solving the big questions. We offer a wide selection of validated Firefly luciferase reporter cell lines that measure transcription factor activity as a read-out for various signaling pathways.
We also offer Gaussia luciferase reporter cell lines, a special type of luciferase that is naturally secreted from cells, which allows for the measurement of reporter activity in the media without harming the cells.
Principle
TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation
Benefits
High Sensitivity & Responsiveness
Each cell line is validated to induce a strong reporter signal in response to stimuli, and is highly responsive to our Firefly Luciferase Substrate.
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Routine Mycoplasma Testing
All cell lines tested negative for mycoplasma.
Consistent
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TF reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
We also offer our own Firefly Luciferase Substrate which can generate a strong light signal that is comparable to our competitor's, but at a more cost-effective price.
Time-Saving
Cell line can be used for experiments right away to study different signaling pathways.
Signaling Pathways
Cell Lines
