Signosis’ luciferase reporter cell lines are engineered to sensitively and specifically monitor transcription factors (TF) transactivation in cells. These stable cell lines are built using a proprietary enhancer construct that contains multiple tandem repeats of a TF-specific cis-regulatory element, ensuring high responsiveness while significantly reducing basal expression that drives luciferase gene expression in response to TF activity.

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To establish each stable line, cells are transfected with TF reporter vector containing Hygromycin or Neomycin resistance gene, followed by antibiotic selection to isolate clones with stably integration. Individual clones are screened for inducibility and consistent responses to extracellular or intracellular signals that activate the target TF. The clone with the highest inducibility is selected and expanded to produce stable cell lines.

 

Signosis’ stable cell lines exhibit low basal expression, high inducibility, and are functionally reliable and reproducible over extended culture, maintaining consistent performance through 25–30 passages.

 

We offer two luciferase reporter systems: Firefly Luciferase and Gaussia Luciferase. Firefly luciferase is a widely used, reliable system that generates a bright, stable signal for gene expression detection. Gaussia luciferase, derived from Gaussia princeps, is a second-generation reporter that is secreted into the culture medium, eliminating the need for cell lysis. This enables non-invasive, continuous monitoring of TF activity while providing higher sensitivity and stability compared to Firefly or Renilla luciferases.