Luciferase Reporter Cell Lines for Monitoring
TF Transactivation
Our Luciferase Reporter Stable cell lines are genetically engineered to express luciferase during the activation of specific Transcription Factors (TFs) Binding and Activation to provide an efficient approach to study and monitor various cellular processes, including gene regulation and signaling pathways.
We offer a wide selection of validated Firefly luciferase reporter cell lines that measure transcription factor activity as a read-out for various signaling pathways.
We also offer Gaussia luciferase reporter cell lines, a special type of luciferase that is naturally secreted from cells, which allows for the easy measurement of reporter activity directly in the media without any need to harvest the cells.
Principle
Signosis’ luciferase reporter cell lines are engineered to sensitively and specifically monitor transcription factor (TF) activity in live cells. Each stable line is built using a proprietary enhancer construct containing multiple tandem repeats of TF-specific cis-regulatory elements — ensuring high inducibility with minimal basal expression.
To generate each line, cells are transfected with a TF-specific reporter vector containing a selection marker (Hygromycin or Neomycin). Following antibiotic selection, individual clones are screened for responsiveness and reproducibility. The most inducible and stable clone is selected and expanded for distribution.
Each cell line is rigorously tested to ensure accuracy, specificity, and reproducibility.
The key aspects of validation and quality guarantees applied to all of our cell lines:
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Consistent, functional reliability through 25–30 passages
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High specificity to the target signaling pathway
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Low background signal combined with strong inducibility
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Mycoplasma‑free, identity‑verified, and morphologically monitored.
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Compatibility with Firefly or Gaussia luciferase systems for flexible assay design
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Benefits
Validated & Quality Assured
All cell lines undergo rigorous validation to ensure accuracy, specificity, and reproducibility.
Stable and Reliable
Reporter expression and cellular behavior remain consistent across multiple passages and freeze-thaw cycles.
Ready-to-use
Cryopreserved cells are delivered assay-ready and can be revived and plated directly for experiments with minimal recovery time.
High Sensitivity & Proven Performance
Reporter cells produce a strong and measurable signal in response to stimulation, with reliable performance across multiple validation experiments.
Fully QC‑Screened
Certified mycoplasma-free and regularly monitored for morphology and health.
Optimized Detection
Compatible with our highly sensitive Luciferase Substrates for accurate and robust signal detection.
High Pathway Specificity
Confirmed through selective ligand stimulation; each cell line responds only to its target signaling pathway with limited to no cross-reactivity.
Backed by Data
A detailed Certificate of Analysis is available upon request, including assay results and QC metrics.
Customizable
We offer custom development of transcription factor (TF) reporter cell lines tailored to your specific pathway or research needs.
Signaling Pathways
Cell Lines
