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Culture-Free Toxicity
Cell Panel Assay

Introduction

The Signosis Culture‑Free Toxicity Cell Panel Assay is a ready‑to‑run 96‑well assay plate pre‑seeded with validated luciferase reporter stable cell lines targeting eight critical stress‑related transcription factor (TF) pathways: XRE, FXR, NRF2, HIF, p53, NFκB, ATF4, and CREB.

 

Designed for toxicity screening, environmental chemical testing, and mechanistic toxicity profiling, this innovative platform eliminates the need for cell seeding or culture maintenance. Simply add your compounds directly to the wells and measure luciferase activity to gain fast, reproducible insights into xenobiotic metabolism, oxidative stress, hypoxia, DNA damage, inflammation, and metabolic regulation..

 

​Explore our full collection of transcription factor (TF) luciferase reporter stable cell lines, including those used in the panel and additional TFs available for individual purchase. These cryopreserved cell lines can be cultured using standard techniques, offering flexibility for custom assay development and mechanistic studies.

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Custom configurations are also available to include other TFs of interest, providing a flexible and standardized solution for stress‑related drug discovery and mechanistic research.​

Principle

The One-Step Environmental Stress & Toxicology Panel uses luciferase reporter stable cell lines to monitor the activation of eight key transcription factor (TF) pathways involved in stress response, toxicology, and xenobiotic metabolism. Each reporter cell line contains a luciferase gene controlled by TF-specific response elements. When a TF is activated, it binds to its response element, inducing luciferase expression that is measured as luminescence.

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  • XRE (Xenobiotic Response Element): Detects activation of the aryl hydrocarbon receptor (AhR) pathway involved in xenobiotic metabolism and detoxification.

  • FXR (Farnesoid X Receptor): Monitors bile acid signaling and its role in metabolic regulation and xenobiotic responses.

  • NRF2 (Nuclear Factor Erythroid 2–Related Factor 2): Reflects the antioxidant response and cellular defense against oxidative stress and electrophilic agents.

  • HIF (Hypoxia-Inducible Factor): Reports on hypoxia-related transcriptional activity controlling oxygen homeostasis and cellular adaptation.

  • p53: Tracks DNA damage response, genotoxic stress, and apoptosis regulation in toxicology studies.

  • NFκB (Nuclear Factor Kappa B): Detects inflammatory and stress-driven transcription related to toxic or environmental insults.

  • ATF4 (Activating Transcription Factor 4): Monitors the integrated stress response (ISR) linked to ER stress, amino acid metabolism, and redox regulation.

  • CREB (cAMP Response Element-Binding Protein): Reports on stress-adaptive signaling associated with energy metabolism and cellular survival mechanisms.

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Application

  • Profiling xenobiotic metabolism and activation of detoxification pathways mediated by TFs like XRE and FXR.

  • Investigating oxidative stress responses through NRF2 signaling under toxic or stressful conditions.

  • Studying cellular adaptation to hypoxia using HIF reporter activity.

  • Assessing DNA damage and genotoxic stress via p53 activation.

  • Evaluating inflammatory and stress signaling pathways involving NF-κB, ATF4, and CREB.

  • Toxicity screening of environmental pollutants, pharmaceuticals, and industrial chemicals for safety and risk assessment.

  • Mechanistic studies of environmental stress-induced cellular dysfunction and disease processes.

  • Supporting drug discovery efforts targeting pathways involved in cellular stress and toxicity.

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Benefits

No Culture Required

Pre‑seeded with luciferase reporter cell lines, ready for immediate testing

Standardized & Reproducible

Consistent cell seeding and validation ensure reliable comparisons

Complete Reagent Package

Includes lysis buffer and luciferase substrate

Covers Eight Core Stress Pathways

Simultaneously analyze 8 key transcription factor (TF) pathways in a single 96-well format for efficient pathway screening.

Highly Sensitive & Quantitative

Direct correlation between TF activation and luminescence signal

Customizable

Option to include additional transcription factors relevant to your research

Fast, One‑Step Workflow

Add compounds and measure luminescence, reducing hands‑on time

Ideal for Multiple Applications

Perfect for antioxidant drug screening, toxicology, stress biology, and mechanistic studies

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