ADP Kinase Activity Kit
Measuring ADP levels in samples is a widely used approach for analyzing kinase activity, as ADP production directly reflects enzymatic phosphorylation events. The Signosis ADP Detection Kit differs from other commercial kits in that it measures pyruvate as the final readout rather than ATP. This unique detection strategy eliminates the need for a complicated ATP depletion step, simplifying the workflow and reducing assay time.
Principle
Signosis’ ADP Detection Kit uses a series of enzyme reactions to measure ADP levels in samples. First, ADP is converted to ATP with pyruvate kinase, which produces pyruvate. Then, the pyruvate is then oxidized by pyruvate oxidase to form a pyruvate byproduct and hydrogen peroxide. The pyruvate, which corresponds to the ADP levels in the original sample, is determined by quantifying the hydrogen peroxide generated by the enzyme reaction with a fluorogenic probe that can be measured with a spectrophotometer.
Benefits
Robust Data
Pyruvate readout bypasses issues from incomplete ATP removal or fluctuating ATP levels in kinase reactions.
Compatible with Multiple Sample Types
Capable of analyzing cells, tissue, blood, and other biological samples.
Improved Efficieny
No ATP-depletion step needed; pyruvate-based detection delivers a simpler workflow than ADP-Glo multi-step protocols.