Signosis’ ADP Detection Kit uses a series of enzyme reactions to measure ADP levels in samples. First, ADP is converted to ATP with pyruvate kinase, which produces pyruvate. Then, the pyruvate is then oxidized by pyruvate oxidase to form a pyruvate byproduct and hydrogen peroxide. The pyruvate, which corresponds to the ADP levels in the original sample, is determined by quantifying the hydrogen peroxide generated by the enzyme reaction with a fluorogenic probe that can be measured with a spectrophotometer.
 
This kit is especially useful for analyzing kinase activity which is commonly assessed by analyzing changes in ADP levels. Since pyruvate is being measured rather than ATP, no ATP depletion step is necessary for this assay, unlike many other commercial ADP detection kits.