CRISPR-Cas technology enables precise genome editing in a wide range of biological systems, offering powerful applications in gene knockout and functional genomic studies. The CRISPR-Cas9 system utilizes a single guide RNA (sgRNA) to direct the Cas9 nuclease to a specific DNA sequence, inducing a precisely targeted double-stranded break in the targeted region of the genomic loci. This break activates the cell's endogenous DNA repair mechanisms, which can be exploited for genome editing purposes. One such pathway, non-homologous end joining (NHEJ), is error-prone and often results in insertions or deletions (indels) at the cleavage site. These indels can disrupt the coding sequence of a gene, effectively "knocking out" its function.

To facilitate efficient gene editing, Signosis offers an In Vitro sgRNA Transcription and Cas9 sgRNA screening manual to allow for both easy synthesis of sgRNA, and to test and screen the efficacy of the designed sgRNA.

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• CRISPR-Cas9 sgRNA IVT kit – In Vitro Transcription of sgRNA

• CRISPR-CAS9 sgRNA Screening kit – In Vitro screening of sgRNA, with Cas9 Nuclease included

• Complete CRISPR-Cas9 sgRNA Screening Kit – Good value of the above two kits combined, allowing for both production of sgRNA and screening of its putative target in vitro.

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