Gaussia Luciferase Reporter Stable Cell Lines for Real-Time Pathway Activation Assays
Signosis’ Gaussia Luciferase Reporter Stable Cell Lines are advanced cell-based assay tools designed for sensitive monitoring of gene expression, signal transduction pathways, transcription factor activity, and drug responses in live cells.
Each stable cell line is engineered to express a secreted Gaussia Luciferase reporter gene under the control of a specific promoter or response element of interest. Upon pathway activation, Gaussia luciferase is secreted directly into the culture medium, allowing researchers to measure reporter activity non-invasively without cell lysis.
Unlike traditional intracellular luciferase systems, Gaussia luciferase reporter assays enable real-time, longitudinal monitoring of cellular responses from the same cell population over multiple time points. This secreted luciferase system significantly simplifies assay workflows, reduces sample handling, preserves cell viability, and improves experimental consistency.
Signosis Gaussia Luciferase Reporter Stable Cell Lines provide:
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High sensitivity and low background
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Non-destructive live-cell monitoring
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Real-time kinetic analysis
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Compatibility with 96-well and 384-well HTS formats
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Robust and reproducible reporter responses
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Convenient repeated measurements without cell harvesting
Gaussia Luciferase Reporter Cell Line Products
Target | Name | SKU | Price (USD$) |
|---|---|---|---|
ER Stress-ERSE | ER Stress-ERSE Secreted Luciferase Reporter HEK293 Stable Cell Line (2 vials) | SL-4068 | $3,870.00 |
NFkB | NFkB Secreted Luciferase Reporter HEK293 Stable Cell Line (2 vials) | SL-4012 | $2,904.00 |
NFkB | NFkB Secreted Luciferase Reporter HeLa Stable Cell Line (2 vials) | SL-4001 | $2,904.00 |
NRF2/ARE | NRF2/ARE Secreted Luciferase Reporter HEK293 Stable Cell Line (2 vials) | SL-4042 | $2,904.00 |
SMAD/BMP | SMAD/BMP Secreted Luciferase Reporter HEK293 Stable Cell Line (2 vials) | SL-4051 | $3,870.00 |
Don't see a particular cell line of interest?
Principle
Signosis' TF Gaussia Luciferase Reporter Stable Cell Lines utilizes a specially engineered promotor construct to express Gaussia Luciferase under the activation of your desired TF of interest. The promoter region consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive Gaussia Luciferase gene expression.
The Gaussia luciferase reporter is derived from the marine copepod Gaussia princeps, which is a naturally secreted protein with a molecular weight of approximately 20 kDa and exhibits exceptionally high luminescence intensity. Its secreted nature allows the reporter enzyme to accumulate directly in the culture medium, eliminating the need for cell lysis during assay procedures.
Gaussia luciferase catalyzes a bioluminescent reaction using a coelenterazine-based substrate to generate light output. To support optimal assay performance, Signosis provides a highly sensitive, one-step Gaussia Luciferase Substrate (SKU: GLUC010) specifically optimized for robust and convenient detection of secreted Gaussia luciferase activity.
