Firefly Luciferase Reporter Cell Lines for Monitoring
TF Transactivation
Signosis offers a comprehensive collection of firefly luciferase reporter cell lines designed for sensitive monitoring of transcription factor (TF) transactivation. Each cell line is engineered to stably express a luciferase reporter under the control of a TF-specific response element, enabling rapid, quantitative assessment of TF activity in cells. These cell lines provide a reliable tool for investigating signaling pathways, validating gene regulatory mechanisms, and assessing cellular responses to drugs or environmental stimuli—without the need for additional transfection steps. Optimized for high-throughput screening, these reporters streamline experimental workflows and improve data reproducibility.
Applications include:
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Gene regulation studies
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Signal transduction pathway analysis
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Compound screening and drug discovery
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Toxicity and stress response assays
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Time-course transcriptional profiling
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Validation of NGS or transcriptomics findings
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Functional characterization of genetic variants or CRISPR edits
Principle
TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation
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Products
Benefits
Validated & Quality Assured
All cell lines undergo rigorous validation to ensure accuracy, specificity, and reproducibility.
Stable and Reliable
Reporter expression and cellular behavior remain consistent across multiple passages and freeze-thaw cycles.
Ready-to-use
Cryopreserved cells are delivered assay-ready and can be revived and plated directly for experiments with minimal recovery time.
High Sensitivity & Proven Performance
Reporter cells produce a strong and measurable signal in response to stimulation, with reliable performance across multiple validation experiments.
Fully QC‑Screened
Certified mycoplasma-free and regularly monitored for morphology and health.
Optimized Detection
Compatible with our highly sensitive Firefly Luciferase Substrate for accurate and robust signal detection.
High Pathway Specificity
Confirmed through selective ligand stimulation; each cell line responds only to its target signaling pathway with limited to no cross-reactivity.
Backed by Data
A detailed Certificate of Analysis is available upon request, including assay results and QC metrics.
Customizable
We offer custom development of transcription factor (TF) reporter cell lines tailored to your specific pathway or research needs.
