Custom ELISA Service
Signosis’s Custom ELISA Services enable you to measure multiple cytokines, proteins, or biomarkers of your choice in a single experiment—compatible with human, mouse, or rat samples. Our flexible, high-throughput platform streamlines protein quantification for studies in inflammation, immune response, and biomarker discovery. With accurate and reproducible results, personalized assay design, and expert data analysis, our ELISA services provide a reliable solution to accelerate your research and optimize experimental efficiency.
Principle
Sandwich ELISA
In this assay, cytokines present in the sample bind to specific capture antibodies pre-coated on a 96-well plate. After unbound components are washed away, biotin-labeled detection antibodies are added to bind the captured cytokines. Streptavidin-HRP is then introduced, forming a complex with the biotin-labeled antibodies.
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Colorimetric measurement: TMB substrate is added, producing a blue color in the presence of HRP. The reaction is stopped with Stop Solution, turning the color yellow. The intensity of the color is measured at an absorbance of 450 nm and is directly proportional to the amount of cytokine present.
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Chemiluminescent measurement: A luminescent HRP substrate is added, and the resulting signal is measured as relative light units (RLUs) using a plate luminometer. The luminescent intensity reflects the concentration of each cytokine.
Our service enables simultaneous and quantitative detection of multiple cytokines in a single experiment.
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Select From Our In-Stock Cytokine Options
Need to measure additional proteins or biomarkers not listed?
No problem—our team can design a customized assay tailored to your research needs.
Benefits
Customizable Multiplex Panels
Select multiple cytokines of your choice for tailored research.
Wide Sample Compatibility
Suitable for serum, plasma, CSF, urine, and tissue homogenates from human, mouse, or rat models
Quantitative Results
Precise quantitative data can be measured with protein standards.
