Human Tumor Marker Kits
Tumor markers are substances found in the body that are produced by cancer cells or healthy cells in response to cancer. These markers provide important information because they can be used to help detect the presence of a cancer during diagnosis and determine the best type of treatment for the cancer. In addition, cancer biomarkers are used by physicians to monitor how a cancer is progressing and responding to a certain treatment. Signosis provides a series of tumor marker ELISA kits, including alpha-fetoprotein and CEACAM5.
Principle
In this assay, the test sample initially reacts with the solid phase capture antibody, resulting in the biomarker protein being bound to the well. The wells are then washed to remove unbound proteins, and HRP-conjugated antibodies are added to bind to the cytokines. After washing away the unbound antibodies, the HRP substrate, TMB, is added and forms a blue color when the HRP-linked antibodies are detected. The reaction is then terminated with Stop Solution, which changes the color from blue to yellow. The biomarker concentration in each well is directly proportional to its color intensity and can be quantified by measuring its optical density at 450 nm (OD450) in a microplate reader.
Benefits
Specific
A pair of highly selective antibodies that bind specifically to the biomarker of interest.
Simple Procedure
An HRP-conjugated detection antibody makes the assay simpler with fewer steps.
Efficient & Flexible
All in one system with a pre-coated plate with twelve 8-well strips.
Cost-Effective Analysis
High quality results at a reasonable price.
