Culture-Free Immune Response Cell Panel (Suspension Format)
Introduction
Understanding the activation of transcription factor (TF) pathways is essential for studying cellular responses, drug effects, and signal transduction mechanisms. To meet this need, Signosis offers our Immune Response Cell Panel, using transiently transfected K562 cells—a suspension human myeloid leukemia cell line ideal for pathway analysis.
Each plate comes pre-loaded with transfected cells and is ready to use—no need for plating or transfection. Simply add your treatments and measure luciferase activity.
This assay utilizes transiently transfected human K562 suspension cells to monitor the activation of eight key transcription factor (TF) signaling pathways: NFκB, NFAT, NRF2, AP1, CREB, STAT3, ATF6, and IRF. Each row of the 96-well plate contains cells transfected with a luciferase reporter plasmid under the control of a specific TF-responsive element, enabling simultaneous analysis of eight distinct signaling pathways across 12 different treatments.
Upon stimulation, activated transcription factors bind to their corresponding response elements within the reporter construct, inducing the expression of firefly luciferase. The level of luciferase activity, quantified using a luciferase substrate in the provided lysis buffer, directly reflects the activation status of the respective pathway.
Explore our full collection of transcription factor (TF) luciferase reporter stable cell lines, including those used in the panel and additional TFs available for individual purchase. These cryopreserved cell lines can be cultured using standard techniques, offering flexibility for custom assay development and mechanistic studies.
Application
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Monitoring key transcription factors (TFs) involved in immune cell activation, differentiation, and function such as NF-κB, STATs, IRFs, and AP-1.
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Profiling immune signaling pathways in various blood cell types including T cells, B cells, macrophages, dendritic cells, and NK cells.
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Studying inflammatory responses and cytokine signaling in autoimmune diseases, infections, and chronic inflammation.
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Evaluating immune modulation effects of drugs, biologics, or small molecules targeting immune-related pathways.
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Investigating transcriptional regulation in hematopoiesis and blood cell lineage commitment.
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Screening potential therapeutics for immuno-oncology, immunodeficiency, and hematological disorders by assessing TF activity changes.
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Understanding molecular mechanisms underlying immune dysregulation in diseases like cytokine storm, sepsis, and autoimmune disorders.
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Supporting biomarker discovery for immune function and blood-related pathologies.
Benefits
No Culture Required
Pre‑seeded with luciferase reporter cell lines, ready for immediate testing
Standardized & Reproducible
Consistent cell seeding and validation ensure reliable comparisons
Complete Reagent Package
Includes lysis buffer and luciferase substrate
Covers Eight Core Stress Pathways
Simultaneously analyze 8 key transcription factor (TF) pathways in a single 96-well format for efficient pathway screening.
Highly Sensitive & Quantitative
Direct correlation between TF activation and luminescence signal
Customizable
Option to include additional transcription factors relevant to your research
Fast, One‑Step Workflow
Add compounds and measure luminescence, reducing hands‑on time
Ideal for Multiple Applications
Perfect for antioxidant drug screening, toxicology, stress biology, and mechanistic studies