The AR Luciferase Reporter MDA-MB-453 stable cell line is a human breast cancer-derived cell line engineered to provide a sensitive and reliable platform for monitoring androgen receptor (AR) signaling activity. This cell line stably expresses firefly luciferase under the control of androgen receptor response elements (ARE), offering a powerful tool to study AR pathway modulation in vitro.
Luciferase expression in this cell line is directly correlated with AR activation, making it an ideal reporter system for quantifying AR pathway activity in response to a variety of stimuli. Whether used for testing androgenic compounds, antiandrogens, AR modulators, or gene functions related to AR signaling, this cell line delivers reproducible and high-throughput results. Activation of AR by ligands such as dihydrotestosterone (DHT) or synthetic agonists leads to a robust luminescent signal, while AR antagonists or inhibitors can suppress luciferase expression.
Key Features:

  
Sensitive, quantitative readout: The luminescence directly correlates with the level of AR activation, allowing for precise analysis.
  
High sensitivity to AR ligands and antagonists: Enables detailed characterization of AR pathway responses, ideal for drug discovery, endocrine disruption studies, and mechanistic AR research.
  
Durable and stable integration: The luciferase reporter construct was integrated via stable transfection and G418 selection, ensuring long-term stability and consistent reporter expression.
  
Robust performance: The most responsive and stable clone—demonstrating a 22-fold induction upon AR activation via DHT—was selected to ensure a high signal-to-background ratio and consistent, reproducible results across experiments.

This cell line retains key characteristics of its parental MDA-MB-453 breast cancer origin, making it suitable for a variety of applications, including drug screening, toxicology studies, and endocrine signaling research. The reporter system is compatible with both manual and automated luminescence detection, offering flexibility for high-throughput screening and mechanistic investigations.