SMAD 1/5/8 Responsive Luciferase Reporter Stable A549 Cell Line is derived from human lung adenocarcinoma, and stably express firefly luciferase reporter gene under the control of SMAD 1/5/8 response element. This cell line is an ideal cellular model for monitoring the activation of BMP response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Bone morphogenic protein (BMP) is involved in embryogenesis, development of many organ systems and adult tissue homeostasis. SMAD (Smad1/5/8) are activated during the signal transduction and then form a complex with SMAD4, translocate into the nucleus where they regulate expression of transcriptional factors and transcriptional coactivators. Deficiency in BMP production or functionality usually leads to marked defects or severe human diseases associated with most organ systems. Monitoring the BMP activity is essential to research of the BMP signaling-associated diseases and conduct drug discovery.
Signosis has established BMP luciferase reporter A549 stable cell line that has been stably transfected with pTA-BMP-luciferase reporter vector, which contains 4 repeats of SMAD1/5/8 binding sites, a minimal promoter upstream of the firefly luciferase coding region. Therefore, the cell line can be used as a reporter system for monitoring the activation of SMAD 1/5/8 triggered by stimuli treatment, enforced gene expression and gene knockdown.
