The SMAD 2/3 Responsive Luciferase Reporter HEK293 Stable Cell Line is derived from human embryonic kidney and stably expresses a firefly luciferase reporter gene under the control of the SMAD 2/3 response element. This cell line serves as an ideal model for monitoring the activation of the TGFβ signaling pathway, enabling the study of cellular responses triggered by various stimuli treatments, gene overexpression, and gene knockdown.
 
SMAD proteins are key transcription factors that respond to transforming growth factor-β (TGFβ) signaling. TGFβ induces its membrane receptors to directly activate SMAD proteins. These activated SMADs then form complexes with SMAD4 (co-SMAD), translocate from the cytoplasm to the nucleus, and bind to target promoter regions to regulate gene transcription. Dysregulation of the TGFβ pathway can lead to immunosuppression and angiogenesis, making cancer more invasive and contributing to other disease processes.
 
Key Features:
Stable Integration: The SMAD luciferase reporter vector, containing SMAD binding sites and a minimal promoter upstream of the firefly luciferase coding region, was stably integrated into HEK293 cells, ensuring consistent and reproducible monitoring of SMAD activation.
Luciferase Activity Assay: The cell line allows for the assessment of SMAD activation by measuring luciferase activity after cell lysis, providing a quantitative readout of TGFβ signaling and transcriptional changes.
Versatile Applications: The SMAD reporter system is ideal for studying the activation of SMAD in response to various stimuli such as TGFβ1, gene overexpression, or gene knockdown. It is useful for high-throughput screening, drug discovery, and functional genomics studies.
Reproducible Results: Hygromycin selection ensures the stable expression of the luciferase reporter, providing reliable performance across experiments.
 
This SMAD/TGFβ Luciferase Reporter HEK293 Stable Cell Line is a powerful tool for studying the TGFβ signaling pathway, SMAD activation, and gene expression, making it invaluable for research in cancer biology, cellular signaling, and drug discovery.