The TLR3/NF-κB Luciferase Reporter HEK293 Stable Cell Line is derived from human embryonic kidney (HEK293) cells and is stably transfected to express full-length human Toll-like receptor 3 (TLR3) together with a firefly luciferase reporter gene under the transcriptional control of an NF-κB response element. The functional activity of this cell line has been validated using a TLR3 ligand assay. Upon stimulation with poly(I:C), TLR3 activation rapidly initiates the TRIF-dependent signaling pathway, leading to nuclear translocation of NF-κB and a corresponding increase in luciferase activity.
 
Toll-like receptor 3 (TLR3) is a pattern recognition receptor of the innate immune system that detects double-stranded RNA (dsRNA), a molecular signature commonly associated with viral infection. TLR3 is primarily localized in endosomal compartments, where it recognizes dsRNA derived from viruses or synthetic analogs such as poly(I:C).
 
The cell line was established by co-transfection with a pCMV-TLR3 expression vector, an NF-κB–driven firefly luciferase reporter vector, and a G418 resistance vector, followed by selection with G418. Hygromycin-resistant clones were subsequently screened for poly(I:C)-induced luciferase activity.