The Ferrozine Iron Assay Kit utilizes ferrozine to detect ferrous iron (Fe²⁺) in biological samples. In this assay, ferrous iron reacts with ferrozine to form a purple-colored complex that can be quantified spectrophotometrically at an absorbance of 562 nm. Ferric iron (Fe³⁺) can also be measured following reduction to Fe²⁺, allowing determination of total iron content. The intensity of the colored complex is directly proportional to the iron concentration in the sample, providing a simple, sensitive, and reliable method for quantifying iron in cell lysates, tissue homogenates, serum, plasma, and other biological samples.