Steroid Hormone ELISA Kits
Signosis’ Steroid Hormone ELISA Kits allow researchers to analyze four critical steroid hormones – cortisol, testosterone, estradiol (E2), and progesterone from any organism including human, mouse, and rat samples. In addition to individual kits, Signosis offers a multiplex combo kit which can measure these four hormones simultaneously on a single 96-well plate. These kits are designed for high sensitivity and specificity, utilizing an ELISA-based platform optimized for serum, cell and tissue lysates, or other biological fluids. This assay is ideal for applications in endocrinology, stress research, reproductive biology, and clinical diagnostics. Whether you're studying hormone regulation, disease progression, or therapeutic response, Signosis' Steroid Hormone ELISA Kits will help accelerate your research.
Principle
In this assay, hormones present in the sample bind to specific capture antibodies pre-coated on a 96-well plate. After unbound components are washed away, biotin-labeled detection antibodies are added to bind the immobilized hormones. Streptavidin-HRP is then introduced, forming a complex with the biotin-labeled antibodies. Then, TMB substrate is added, producing a blue color in the presence of HRP. After color development, the reaction is stopped with Stop solution, changing the blue color to yellow. The intensity of the color is measured at an absorbance of 450 nm and is used to determine the hormone level in the sample.
This method enables simultaneous and quantitative detection of multiple hormones in a single assay.
Products
Hormone | Product | SKU | Price (USD$) |
|---|---|---|---|
Multiplex | EA-7070 | 1100 | |
Cortisol | EA-7071 | 400 | |
Testosterone | EA-7072 | 400 | |
Estradiol | EA-7073 | 400 | |
Progesterone | EA-7074 | 400 |
Benefits
Multiplex
One ELISA plate can analyze 4 different steroid hormones simultaneously.
Compatible with Multiple Sample Types
Suitable for serum, plasma, cell and tissue lysates, and other sample types.
Quantitative Profiling & Analysis
Allows quantitative analysis of hormone levels in samples. Hormone standards are available for purchase separately.
