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Caspase Detection Assay Kit
Signosis’s Caspase Assay Kit provides a comprehensive solution for measuring key caspases in different sample types including blood, cells, and tissue. This kit can measure the activity of caspase-3/7, caspase-8, and caspase-9 by utilizing a unique fluorogenic substrate to detect each protease. A multiplex kit is also available which can measure all three caspase types simultaneously in the same sample.
Principle
Singleplex
Each assay utilizes a unique fluorogenic substrate that is specifically cleaved by each caspase variant. Once cleaved, the substrate produces a luminescent signal which can be detected with a fluorescence plate reader.
Caspase-3/7: Ac-DEVD-AFC (Ex/Em: 400nm/505nm)
Caspase-8: Ac-IETD-AFC (Ex/Em: 400nm/505nm)
Caspase-9: Ac-LEHD-AFC (Ex/Em: 345nm/445nm)
Multiplex
In this assay, the substrates for each caspase type have unique fluorogenic dyes with different emission spectra, which allows for multiplex detection of multiple caspase types in the same sample.
Caspase-3/7: Ac-DEVD-AMC (Ex/Em: 344nm/440nm)
Caspase-8: Ac-IETD-AFC (Ex/Em: 400nm/505nm)
Caspase-9: Ac-LEHD-R110 (Ex/Em: 500nm/522nm)
Scale | Product | SKU | Price (USD$) |
|---|---|---|---|
Multiplex | EA-7021 | 700 | |
Singleplex | EA-7020 | 400 |
Benefits
High Sensitivity
Fluorescence spectroscopy provides higher sensitivity than colorimetric methods.
Measure Multiple Caspases
Kit includes probes for three different caspase types.
Compatibility
The assay is suitable for any biological liquid material such as cell lysate, blood, and tissue homogenate.
Multiplex Capability
Measure multiple caspase types simultaneously in the same sample.
