SMAD 1/5/8 Responsive Secreted Luciferase Reporter HEK293 Cell Line is derived human embryonic kidney, and stably express firefly luciferase reporter gene under the control of SMAD 1/5/8 response element. This cell line is an ideal cellular model for monitoring the activation of BMP response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
 
Bone morphogenic protein (BMP) is involved in embryogenesis, development of many organ systems and adult tissue homeostasis. Smads (Smad1/5/8) are activated during the signal transduction and then form a complex with Smad4, translocate into the nucleus where they regulate expression of transcriptional factors and transcriptional coactivators. Deficiency in BMP production or functionality usually leads to marked defects or severe human diseases associated with most organ systems. Monitoring the BMP activity is essential to research of the BMP signaling-associated diseases and conduct drug discovery.
 
Secreted reporter stable cell lines are powerful tools in modern biological research, offering unique advantages for studying gene expression and cellular signaling pathways. These cell lines are engineered to stably express a secreted luciferase reporter gene, typically under the control of a specific promoter or response element of interest. The secreted nature of the reporter protein allows for non-invasive, real-time monitoring of gene activation without the need for cell lysis. This enables researchers to perform continuous, long-term studies and repeated measurements from the same cell population. The high sensitivity of secreted luciferases, such as Gaussia luciferase, allows for detection of even subtle changes in gene expression.
 
These stable cell lines are particularly valuable for high-throughput screening, drug discovery, and the study of various cellular pathways, offering a streamlined workflow and compatibility with multiple downstream analyses. Their ability to provide dynamic, quantitative data on gene activation makes them an indispensable asset in fields ranging from cancer research to toxicology studies.