Obesity Hormone/Adipokine ELISA Kits
Signosis’ Obesity Hormone/Adipokine ELISA Kits allow researchers to analyze critical hormones and proteins involved in the regulation of metabolism, including insulin, amylin, glucagon, GLP-1, leptin, adiponectin, IL-6, and TNFα. These kits are designed for high sensitivity and specificity, utilizing an ELISA-based platform optimized for serum, cell and tissue lysates, or other biological fluids. These assays are ideal for applications in medicine, pharmacology, and biotechnology, and can be used to study samples from human, mouse, and rat models. In addition to individual kits, Signosis offers a multiplex combo kit which can measure these four hormones simultaneously on a single 96-well plate. Whether you're studying diabetes, obesity, or other metabolic diseases, Signosis' Obesity Hormone/Adipokine ELISA Kits will provide precise and efficient results to accelerate your research.
Principle
This assay utilizes the sandwich ELISA format to measure hormones levels in samples. Hormones present in the sample bind to specific capture antibodies pre-coated on a 96-well plate. After unbound components are washed away, biotin-labeled detection antibodies are added to bind the immobilized hormones. Streptavidin-HRP is then introduced, forming a complex with the biotin-labeled antibodies. Then, TMB substrate is added, producing a blue color when it interacts with HRP. After the blue color fully develops, the reaction is terminated with an acidic Stop solution, which changes the blue color to yellow. The intensity of the yellow color is measured at an absorbance of 450 nm and is used to determine the hormone level in the sample.
The assay can be used to analyze serum, cell, tissue, and other liquid samples from human, mouse, and rat models.
The Obesity Hormone/Adipokine ELISA Combo Kit includes 8 analytes on a single 96-well plate (12 wells per analyte) and enables simultaneous and quantitative detection of multiple targets in a single assay.
Limit of Detection
• Insulin: 1–10 pg/mL
• Amylin: 1–10 pg/mL
• Glucagon: 1–10 pg/mL
• GLP-1: 1–10 pg/mL
• Leptin: 1–10 pg/mL
• Adiponectin: 1–10 pg/mL
• IL-6: 1–10 pg/mL
• TNFα: 1–10 pg/mL
Products
Hormone | Product | SKU | Price (USD$) |
|---|---|---|---|
Multiplex | EA-7084 | 1000 | |
Insulin | EA-7080 | 450 | |
Amylin | EA-7083 | 450 | |
Glucagon | EA-7081 | 450 | |
GLP-1 | EA-7082 | 450 |
Benefits
Efficient and Flexible
Multiple samples can be analyzed simultaneously in a 8x12 strip plate format.
Compatible with Multiple Sample Types
Suitable for serum, plasma, cell and tissue lysates, and other sample types.
Quantitative
Analysis
Includes standards for quantitative analysis of analyte levels in samples.
