Oxidative Damage
Assay Kits
Signosis’ Oxidative Damage ELISA Combo Kit provides a comprehensive, plate based solution for assessing long-term and systemic oxidative injury in models of cardiovascular disease, metabolic disorders, neurodegeneration, aging, and therapeutic intervention studies. This multiplex 96 well assay simultaneously quantifies three key damage biomarkers—8-OHdG (oxidative DNA damage), 8-iso-PGF2α (lipid peroxidation), and OxLDL (lipoprotein oxidation)—from the same sample, offering an integrated view of cumulative oxidative damage across biomolecule classes. Used downstream of ROS and antioxidant measurements, the kit enables researchers to confirm whether candidate interventions actually reduce DNA and lipid damage in the very same samples, providing stronger, mechanism linked evidence of efficacy in preclinical and translational studies.
Principle
Signosis' Oxidative Damage ELISA Combo Kit is a multiplex assay designed to simultaneously measure key biomarkers of oxidative damage: 8-OHdG, 8-iso-PGF2α, and OxLDL in biological fluids such as urine, plasma/serum, or cell media. For analyzing cell and tissue lysates, additional procedures are necessary for preparing the sample and we recommend using the single marker kits (EA-7085, EA-7086) for this purpose.
Competitive ELISA
The 8-OHdG and 8-iso-PGF2α assays use the competitive ELISA format, which is ideal for small-molecule biomarkers lacking multiple epitopes for sandwich detection. Plates are pre-coated with marker-protein conjugates that compete with free markers in samples for binding to specific anti-marker rabbit antibodies. After washing away unbound material, HRP reagent binds to the antibodies bound to the plate. TMB substrate generates a blue color proportional to bound antibody, turning yellow upon acidification. Absorbance at 450 nm is inversely proportional to sample marker concentration—higher free marker levels block the antibody from binding to the plate.
Sandwich ELISA
OxLDL is measured using the sandwich ELISA format. OxLDL in the sample binds to capture antibodies pre-coated on the plate. After washing, biotinylated detection antibody binds to the captured OxLDL on the plate. HRP reagent and TMB substrate produce a blue color proportional to OxLDL concentration in the sample. Acidic stop solution turns the solution color yellow, and absorbance at 450 nm directly correlates with OxLDL levels in the sample.
Products
Marker | Product | SKU | Price (USD$) |
|---|---|---|---|
Multiplex | EA-7088 | 1500 | |
8-OHdG | EA-7085-A | 580 | |
8-OHdG | EA-7085-B | 630 | |
8-iso-PGF2α | EA-7086 | 580 | |
OxLDL | EA-7087 | 580 | |
DNA Extraction | EA-7089 | 300 |
Benefits
Streamlined Workflow
Includes all necessary reagents for simple and efficient analysis.
Multiplex Capability
Measure multiple oxidative damag markers simultaneously from the same sample.
Compatible with Multiple Sample Types
Specialized protocols are designed to analyze urine, plasma/serum, cells, and tissue samples.
High Sensitivity
Accurately detect low-abundance analytes for precise measurements.