Oxidative Damage
Assay Kits
Signosis’ Oxidative Damage ELISA Combo Kit provides a comprehensive, plate based solution for assessing long-term and systemic oxidative injury in models of cardiovascular disease, metabolic disorders, neurodegeneration, aging, and therapeutic intervention studies. This multiplex 96 well assay simultaneously quantifies three key damage biomarkers—8-OHdG (oxidative DNA damage), 8-iso-PGF2α (lipid peroxidation), and OxLDL (lipoprotein oxidation)—from the same sample, offering an integrated view of cumulative oxidative damage across biomolecule classes. Used downstream of ROS and antioxidant measurements, the kit enables researchers to confirm whether candidate interventions actually reduce DNA and lipid damage in the very same samples, providing stronger, mechanism linked evidence of efficacy in preclinical and translational studies.
Principle
Signosis' Oxidative Damage ELISA Combo Kit is a multiplex assay designed to simultaneously measure key biomarkers of oxidative damage: 8-OHdG, 8-iso-PGF2α, and OxLDL. Each marker is detected by either the competitive ELISA or sandwich ELISA method.
Competitive ELISA
The markers 8-OHdG and 8-iso-PGF2α are analyzed using a competitive ELISA assay. In this assay, the plate is coated with the target marker conjugated to a protein, which competes with the free markers in the samples that are added to the plate. After the samples containing free marker are added to the plate, an anti-marker rabbit antibody is added to the samples and binds to both the free markers and conjugated markers immobilized on the plate. The plate is then washed to remove any sample and antibodies not binding to the markers bound on the plate. The antibodies that are bound to the immobilized markers on the plate are measured by adding anti-rabbit IgG conjugated to HRP, which binds to the rabbit antibodies. To detect the HRP, TMB substrate is added, which produces a blue color when it interacts with HRP. After the blue color fully develops, the reaction is terminated with an acidic Stop solution, which changes the blue color to yellow. The intensity of the yellow color is measured at an absorbance of 450 nm and is used to determine the marker level in the sample. Because this is a competitive ELISA assay, the signal is inversely proportional to the marker concentration, since higher free marker concentrations will prevent the labeled antibody from binding to the plate.
Sandwich ELISA
OxLDL is analyzed using a sandwich ELISA assay. In this assay, peptides present in the sample bind to specific capture antibodies pre-coated on a plate. After unbound components are washed away, biotin-labeled detection antibodies are added to bind the immobilized markers. Streptavidin-HRP is then introduced, forming a complex with the biotin-labeled antibodies. Then, TMB substrate is added, producing a blue color when it interacts with HRP. After the blue color fully develops, the reaction is terminated with an acidic Stop solution, which changes the blue color to yellow. The intensity of the yellow color is measured at an absorbance of 450 nm and is used to determine the marker level in the sample.
Products
Marker | Product | SKU | Price (USD$) |
|---|---|---|---|
Multiplex | EA-7088 | 1100 | |
8-OHdG | EA-7085 | 450 | |
8-iso-PGF2α | EA-7086 | 450 | |
OxLDL | EA-7087 | 450 |
Benefits
Streamlined Workflow
Includes all necessary reagents for simple and efficient analysis.
Multiplex Capability
Measure multiple oxidative damag markers simultaneously from the same sample.
Compatible with Multiple Sample Types
Specialized protocols are designed to analyze cells, tissue, or blood/biological fluid samples.
High Sensitivity
Accurately detect low-abundance analytes for precise measurements.
