8-Hydroxy-2′-deoxyguanosine (8-OHdG) is a key biomarker of oxidative DNA damage and an important indicator of cellular oxidative stress. It forms when reactive oxygen species attack the guanine bases within DNA, leading to structural modifications that can disrupt genetic stability. Elevated levels of this molecule reflect increased oxidative burden and impaired antioxidant defenses. Understanding and monitoring 8-OHdG is therefore essential for advancing research into disease mechanisms, evaluating antioxidant therapies, and developing strategies to promote long-term cellular health.
 
Signosis' 8-OHdG ELISA Kit utilizes a competitive ELISA assay to measure 8-OHdG levels in cell and tissue samples. This kit should be used with the DNA Damage Extraction Kit (EA-7089) to isolate DNA from cell or tissue samples so that 8-OHdG can be accurately measured.