The Signosis Oxidative Stress Combo Assay Cell Kit provides a comprehensive solution for measuring key oxidative stress and antioxidant markers in cells in a single, streamlined workflow. This kit enables the detection of reactive oxygen species (ROS), lipid peroxidation (MDA), glutathione (GSH), and superoxide dismutase (SOD) activity by integrating multiple assays into a single kit.
 
First, the DCFDA assay is used to quantify ROS levels in live cells in culture media. After the measuring the ROS levels, the cells are lysed, and the lysates are used to measure MDA levels, GSH levels and SOD activity.
 
Data Analysis & Interpretation
High ROS/RNS + Low GSH/GSSG + High MDA → Oxidative stress damage.
High ROS but High SOD + High GSH/GSSG → Active antioxidant response.
Low SOD + Low GSH/GSSG + High MDA → Severe oxidative damage, impaired defenses.
 
Advantages of This Multiplex Assay
✔ Comprehensive: Measures both oxidative damage & antioxidant defenses.
✔ Efficient: Reduces the need for multiple sample preparations.
✔ High-Throughput Ready: 96-well format for screening compounds or treatments.