Signosis’ Cellular Metabolism Combo Assay Kits provides a convenient and efficient multiplexed solution for the simultaneous quantification of four key components in cellular metabolism: glucose, lactate, ATP, and NADH.

This assay utilizes specific enzymatic reactions for each target, with detection based on either fluorogenic or colorimetric signals that can be measured using a standard microplate reader or a spectrophotometer, enabling high-throughput metabolic profiling in biological samples such as serum, plasma, or cell lysates.

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• Glucose – glucose oxidase catalyzes glucose oxidation, generating H₂O₂, which is detected with a fluorogenic probe.

• Lactate – lactate oxidase converts lactate to pyruvate and H₂O₂, which is detected with a fluorogenic probe.

• Pyruvate – pyruvate oxidase oxidizes pyruvate to acetyl phosphate and H₂O₂, which is detected with a fluorogenic probe.

• ATP – ATP is consumed by glycerol kinase to convert glycerol into glycerol-3-phosphate, which is oxidized to generate H₂O₂, detected with a fluorogenic probe.

• NADH – directly reduces WST-8 to a yellow formazan dye, measured by absorbance at 450 nm.

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Additionally, individual kits for each component (glucose, lactate, pyruvate, ATP, and NADH) are also available for customers who prefer to test specific metabolic markers.