Signosis’ Cellular Metabolism Combo Assay Kit measures key components of cellular metabolism—glucose, lactate, ATP, and NAD⁺/NADH—in blood, biological fluids, cells, or tissue samples. The kit performs up to 100 assays for each analyte using enzyme-based reactions that generate colorimetric or fluorescent signals directly proportional to metabolite levels. Glucose and lactate are oxidized by their respective enzymes to produce hydrogen peroxide, which reacts with a fluorogenic probe for detection. ATP is measured through coupled enzymatic reactions that link ATP consumption to hydrogen peroxide generation, while NAD⁺/NADH levels are determined through a color-forming reaction with the WST-8 dye, enabling fast and reliable quantification.
 
Lysis buffer is available for purchase separately, if needed.