Signosis' Gene Amplification Reagents are designed to improve the efficiency and accuracy of molecular amplification techniques.

• One Step RT-qPCR Assay Kit: This kit combines reverse transcription and qPCR in a single reaction, streamlining workflows while maintaining high accuracy. The kit’s buffer is optimized for seamless cDNA synthesis followed by quantitative PCR, making it ideal for gene expression analysis and detection of low-abundance targets.

• qPCR SYBR Green Master Mix: This master mix is designed for quantitative PCR, using SYBR Green to stain double-stranded DNA. The mix includes all essential components for efficient amplification, ensuring fast and reliable results.

• Enzyme Reagents:

  • Hi-T7 RNA Polymerase is used for RNA synthesis, enabling efficient transcription in vitro.

  • Heat-labile Uracil-DNA Glycosylase prevents carryover contamination by degrading uracil-containing DNA.

  • HS-Taq DNA Polymerase is a high-fidelity enzyme that ensures accurate amplification in qPCR reactions.

  • Bsu DNA Polymerase (Large Fragment) offers high processivity for high-efficiency amplification.

  • M-MLV Reverse Transcriptase (RNase H-) provides high-quality reverse transcription without RNase H activity, making it suitable for cDNA synthesis.

• ROX Reference Dyes: The ROX reference dyes (High and Low) are used to normalize fluorescent signals, ensuring consistent detection across different qPCR instruments.