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Bsu DNA Polymerase, Large Fragment is a DNA polymerase derived from Bacillus subtilis (Bsu) that is expressed through recombinant expression in Escherichia coli. This enzyme is a deletion mutant (1-296 aa deletion) of Bsu DNA Polymerase I, which exhibits 5'-3' DNA polymerase activity but lacks 3'-5' and 5'-3' exonuclease activities. The Bsu DNA Polymerase Large Fragment possesses strand displacement activity and is commonly used in Recombinase Polymerase Amplification (RPA), with the typical amplification temperature being 37°C.

 

**This product does not contain endonucleases, exonucleases, or ribonucleases.

Product Name: Bsu DNA Polymerase, Large Fragment Kit

 

Contents:


- Bsu DNA Polymerase, Large Fragment (BSUP-150, BSUP-750)
- 10× Bsu Reaction Buffer

 

Specifications:


- Bsu DNA Polymerase Size: 50µL with 150µL of 10x BSU Reaction Buffer (BSUP-150),  250µL with 750 µL of 10x BSU Reaction Buffer (BSUP-750)
- Bsu DNA Polymerase Units: 5 U/µL
- Bsu DNA Polymerase Reactions: 50, 250 reactions
 

 

 

Product Applications: Isothermal amplification methods like RPA; RPA strand displacement DNA synthesis; Random primer labeling; cDNA second strand synthesis; Single dA tailing.

 

Storage Conditions: Store at -20°C for two years; transport at ≤0°C.

 

Enzyme Storage Buffer

40 mM Tris-HCl (pH 7.5)

50 mM NaCl

0.1 mM EDTA

1 mM DTT

50% glycerol.

 

10×Bsu Buffer

100mM Tris-HClpH7.9

500mM NaCl

100mM MgCl2

10mM DTT

0.05% Proclin150

 

 

Definition of Activity: One unit (U) of activity is defined as the amount of enzyme required to incorporate 10 nmol of total nucleotides into an acid-insoluble form within 30 minutes at 37°C. Inactivation Incubate at 75°C for 20 minutes.

 

Quality Control Exonuclease Activity Assay: Incubate 5 U of the enzyme with 1 μg of Hind III-treated λDNA at 37°C for 10 hours. No changes should occur in the DNA electrophoresis pattern.

 

Endonuclease Activity Assay: Incubate 5 U of the enzyme with 1 μg of pUC19 plasmid DNA at 37°C for 1 hour. No changes should occur in the DNA electrophoresis pattern.

 

Ribonuclease Activity Assay: Incubate 5 U of the enzyme with 1 μg of 16S, 23S rRNA at 37°C for 1 hour. No changes should occur in the RNA electrophoresis pattern.

 

Please see manual for more information.


 

Bsu DNA Polymerase, Large fragment (Enzyme Included)

$310.00Price

* For large quantity order volume of 10, 20, 30, 100, or 300+, please contact us for a special rate below.