The cell line was generated by lentiviral transduction of Ba/F3 cells with a lentiviral construct encoding human ERBB2 (HER2) P780_Y781insGSP, enabling stable expression of the mutant HER2 protein. Successfully transduced cells were selected and expanded, and HER2 gene integration and protein expression were confirmed by molecular and protein-based analyses.
The cell line can be used for studying the ErbB/HER signaling pathway and for screening or evaluating HER2-targeted drugs and related compounds. The established cell line is mycoplasma-free and provides a stable lentiviral-transduced model for research on oncogenic HER2 signaling and therapeutic development.