Description(s):
The proto-oncogene human epidermal growth factor receptor 2 (HER2) gene also known as ERBB2 is a member of the ERBB/HER RTK family which is comprised of EGFR (EGFR/HER1/ERBB1), HER3/ERBB3, and HER4/ERBB4. This gene encodes for a transmembrane glycoprotein receptor with an intracellular domain with tyrosine kinase activity which is responsible for the activation of intracellular signal transduction pathways controlling epithelial cell growth, differentiation, and angiogenesis. Aberrations of the HER2 gene which usually leads to increased receptor signaling have been observed in many different types of cancer.
 
Signosis' HER2 A775_G776insYVMA/C805S Ba/F3 cell line is a stable cell line generated by lentiviral transduction. The ERBB2 (HER2) A775_G776insYVMA mutation results in the insertion of four amino acids within the kinase domain of the HER2 protein between amino acid residues 775 and 776. The ERBB2 (HER2) C805S mutation is also located within the kinase domain and has been associated with resistance to HER2-targeted therapies.
 
The cell line can be used for studying ErbB/HER signaling pathway.
The cell line can be used for screening or evaluating related drugs/compounds.
Transfected Her2 gene sequence and its expressed protein have been confirmed.
The cell line is mycoplasma-free.