Nicotinamide adenine dinucleotide (NAD⁺) is a vital coenzyme found in all living cells, where it plays a central role in energy metabolism, redox balance, and cellular survival. In addition to its metabolic functions, NAD⁺ acts as a key signaling molecule and essential cofactor for enzymes like sirtuins and PARP, which are crucial for DNA repair, stress response, and longevity. The Signosis NAD⁺ Enzyme Combo Assay Kit enables researchers to simultaneously quantify NAD⁺ levels and the activities of three major NAD⁺-dependent enzymes—SIRT (sirtuins), NAMPT (nicotinamide phosphoribosyltransferase), and PARP (poly(ADP-ribose) polymerase)—in cell and tissue samples. This integrated approach provides a comprehensive tool for studying NAD⁺ biology and its regulatory pathways.
 
This kit includes enough reagents for 100 Assays for each analyte.