Nicotinamide phosphoribosyltransferase (NAMPT) is a crucial enzyme involved in the biosynthesis of NAD⁺, a vital coenzyme in cellular metabolism. NAMPT has been implicated in various biological processes, including inflammation, cellular metabolism, and cancer.
 
The NAMPT Activity Assay kit can measure the activity of NAMPT for compound screening and profiling experiments. This assay detects NAMPT activity through a series of enzyme reactions. First, NAMPT converts nicotinamide (NAM) and PRPP into nicotinamide mononucleotide (NMN). Then NMN is converted by NMNAT into NAD⁺. Lastly, NAD⁺ is reduced to NADH by alcohol dehydrogenase (ADH) in the presence of ethanol. The NADH is detected with a WST solution which reacts with NADH to product a yellow color that can be measured at an absorbance of 450 nm with a plate reader.