Signosis' Dual Luciferase Assay System provides a sensitive, easy-to-use solution for measurement of Firefly and Renilla luciferase activities. This all-in-one kit includes optimized Firefly and Renilla substrates along with a high-quality lysis buffer for efficient cell lysis and rapid detection of both Firefly and Renilla Luciferase activity from a single sample.
 
The assay begins by measuring Firefly luciferase activity. The sample lysate is first mixed with the Firefly substrate, producing a strong luminescent signal. Following Firefly measurement, the Renilla substrate is then added, simultaneously quenching the Firefly while activating the Renilla Luciferase. This allows rapid, sensitive measurement in the same sample without interference.
 
Designed for simplicity and speed, the system is compatible with both manual and automated luminometers for efficient processing of multiple samples and high-throughput assays.