EGFR Vectors
We provide fully sequenced EGFR expression vectors (pMSCV-EGFR-GFP, pCMV-EGFR, and pLenti-MSCV-EGFR-GFP) for gene function analysis and assay development. Our tools are designed for reliability, reproducibility, and fast integration into your research workflow.
pCMV-EGFR Vectors
Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in the EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.
Downstream effects of EGFR activation include modulation of the RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.
We offer a collection of unique cloning and expression cDNA vectors containing the wild-type or mutated EGFR gene. These mutations are clinically approved and are being studied by many researchers. Our high-quality vectors are fully sequenced and ready to use. We also offer custom cloning services for your specific construct needs.
pMSCV-EGF-GFP Vectors Research Application
-
Overexpressing EGF in cells to study the effects of EGF on cell proliferation, differentiation, and survival.
-
Generating stable cell lines expressing EGF for long-term studies of EGF signaling pathways and downstream effects.
-
Investigating the mechanisms of resistance to EGF inhibitors or combination therapies in cells that overexpress EGF.
-
Developing animal models of EGF-driven cancers by transfecting cells with pMSCV-EGF-GFP vectors and injecting them into animals.
-
Evaluating the efficacy of various inhibitors or combination therapies targeting EGF in vitro and in vivo.
pCMV-EGFR Vectors' Principle
Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in the EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.
Downstream effects of EGFR activation include modulation of the RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.
We offer a collection of unique cloning and expression cDNA vectors containing the wild-type or mutated EGFR gene. These mutations are clinically approved and are being studied by many researchers. Our high-quality vectors are fully sequenced and ready to use. We also offer custom cloning services for your specific construct needs.
Benefits
RT Research
Our engineered expression cell lines are perfect for studying RTKs and their pathways, or any other relevant molecular or relevant mechanisms.
Double Selection
Single clones are selected with GFP and hygromycin, which can be used to maintain RTK expression.
Comprehensive Validation
The selected clones have been validated with PCR-sequencing and Western blot analysis.
Functional Analysis
Proliferation tests have been performed on the cell lines with their corresponding inhibitors.
Benefits
Panel of EGFR Mutant Vectors
The panel includes more than 12 ready-to-use EGFR mutants.
Easy Detection
The DYKDDDDK-tag is engineered downstream of the EGFR gene to enable detection by Western blot.
Stable Cell Line Establishment
A hygromycin-resistance gene inserted in the vector can be used for the selection of cells to create a stable cell line.
Custom Service Available
Custom services are available for any mutants that are not listed in our panel of EGFR vectors.
pLenti-MSCV-EGFR-GFP Vectors
Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain. Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.
We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.
pCMV-EGFR Vectors Research Application
-
Overexpressing EGFR in cells to study the effects of EGFR on cell proliferation, differentiation, and survival.
-
Generating stable cell lines expressing EGFR for long-term studies of EGFR signaling pathways and downstream effects.
-
Investigating the mechanisms of resistance to EGFR inhibitors or combination therapies in cells that overexpress EGFR.
-
Developing animal models of EGFR-driven cancers by transfecting cells with pCMV-EGFR vectors and injecting them into animals.
-
Evaluating the efficacy of various inhibitors or combination therapies targeting EGFR in vitro and in vivo.
pLenti-MSCV-EGFR-GFP Vectors
Benefits
Infect both dividing and nondividing cells
DYKDDDDK-tag engineered to downstream of EGFR gene for Western blot
Super bright CopGFP under EF-1 promoter as a selection marker
Contain HIV-1 LTRs and the lentiviral packaging signal (Ψ)
Specific elements (WPRE, cPPT, RRE) included for improving transgene expression
pLenti-MSCV-EGFR-GFP Vectors
Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain. Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.
We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.
pLenti-MSCV-EGFR-GFP Vectors Research Application
-
Generating stable cell lines expressing EGFR for long-term studies of EGFR signaling pathways and downstream effects.
-
Investigating the effects of EGFR mutations on downstream signaling pathways and cellular processes, such as cell proliferation, differentiation, and survival.
-
Identifying novel therapeutic strategies for targeting EGFR in cancer by testing the efficacy of various inhibitors or combination therapies.
-
Screening for small molecule compounds or antibodies that selectively target EGFR mutants over wild-type EGFR.
-
Developing animal models of EGFR-driven cancers by transfecting cells with pLenti-MSCV-EGFR-GFP vectors and injecting them into animals.
-
Evaluating the potential of EGFR and its mutants as diagnostic and prognostic biomarkers for specific types of cancer.
Benefits
EGFR under MSCV promoter for high expression in hematopoietic or stem cells
Super bright CopGFP under EF-1 promoter as a selection marker
DYKDDDDK-tag engineered to downstream of EGFR gene for Western blot
Mutation | Product | SKU | Price (USD$) |
|---|---|---|---|
L858Q | EV-0006 | 1529 | |
Exon 19 Insertion | EV-0010 | 1529 | |
L792H, L858R | EV-0019 | 1911 | |
T790M, L792H, L858R | EV-0020 | 1911 | |
L718Q, L858R | EV-0017 | 1911 | |
L858R | EV-0007 | 1529 | |
T790M, C797S, Exon 19 Deletion | EV-0014 | 1911 | |
G719S | EV-0002 | 1529 | |
WT | ELM-0001 | 1571 | |
T790M, L792H, L858R | ELM-0009 | 1935 | |
A767_dupASV, C797S | ELM-0002 | 1935 | |
H773_V774insH, C797S | ELM-0005 | 1935 |
