EGFR Vectors

pMSCV-EGF-GFP Vectors

pCMV-EGFR Vectors

pLenti-MSCV-EGFR-GFP Vectors

pCMV-EGFR Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.

Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.

pMSCV-EGF-GFP Vectors Research Application

Overexpressing EGF in cells to study the effects of EGF on cell proliferation, differentiation, and survival.
Generating stable cell lines expressing EGF for long-term studies of EGF signaling pathways and downstream effects.
Investigating the mechanisms of resistance to EGF inhibitors or combination therapies in cells that overexpress EGF.
Developing animal models of EGF-driven cancers by transfecting cells with pMSCV-EGF-GFP vectors and injecting them into animals.
Evaluating the efficacy of various inhibitors or combination therapies targeting EGF in vitro and in vivo.

pCMV-EGFR Vectors

EGFR mutations fig created 12-11-2017.jpg

Benefits

Panel of EGFR

Mutant Vectors

The panel includes more than 12 ready-to-use EGFR mutants.

DYKDDDDK-Tag for

Easy Detection

This tag is engineered to downstream of EGFR gene to facilitate Western blot.

Perfect for Stable Cell Line Establishment

The hygromycin-resistant gene is embedded in the vector for stable cell line establishment.

Custom Service Available

We also offer the custom service with the same list price. If you have any mutants in your mind, please let us know.

pLenti-MSCV-EGFR-GFP Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain. Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.

pCMV-EGFR Vectors Research Application

Overexpressing EGFR in cells to study the effects of EGFR on cell proliferation, differentiation, and survival.
Generating stable cell lines expressing EGFR for long-term studies of EGFR signaling pathways and downstream effects.
Investigating the mechanisms of resistance to EGFR inhibitors or combination therapies in cells that overexpress EGFR.
Developing animal models of EGFR-driven cancers by transfecting cells with pCMV-EGFR vectors and injecting them into animals.
Evaluating the efficacy of various inhibitors or combination therapies targeting EGFR in vitro and in vivo.

pLenti-MSCV-EGFR-GFP Vectors

Benefits

Infect both dividing and nondividing cells

DYKDDDDK-tag engineered to downstream of EGFR gene for Western blot

Super bright CopGFP under EF-1 promoter as a selection marker

Contain HIV-1 LTRs and the lentiviral packaging signal (Ψ)

Specific elements (WPRE, cPPT, RRE) included for improving transgene expression

pLenti-MSCV-EGFR-GFP Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain. Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.

pLenti-MSCV-EGFR-GFP Vectors Research Application

Generating stable cell lines expressing EGFR for long-term studies of EGFR signaling pathways and downstream effects.
Investigating the effects of EGFR mutations on downstream signaling pathways and cellular processes, such as cell proliferation, differentiation, and survival.
Identifying novel therapeutic strategies for targeting EGFR in cancer by testing the efficacy of various inhibitors or combination therapies.
Screening for small molecule compounds or antibodies that selectively target EGFR mutants over wild-type EGFR.
Developing animal models of EGFR-driven cancers by transfecting cells with pLenti-MSCV-EGFR-GFP vectors and injecting them into animals.
Evaluating the potential of EGFR and its mutants as diagnostic and prognostic biomarkers for specific types of cancer.