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EGFR Vectors

We provide fully sequenced EGFR expression vectors (pMSCV-EGFR-GFP, pCMV-EGFR, and pLenti-MSCV-EGFR-GFP) for gene function analysis and assay development. Our tools are designed for reliability, reproducibility, and fast integration into your research workflow.

pCMV-EGFR Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in the EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.  

 

Downstream effects of EGFR activation include modulation of the RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

​

We offer a collection of unique cloning and expression cDNA vectors containing the wild-type or mutated EGFR gene. These mutations are clinically approved and are being studied by many researchers. Our high-quality vectors are fully sequenced and ready to use. We also offer custom cloning services for your specific construct needs.​​

pMSCV-EGF-GFP Vectors Research Application

​

  • Overexpressing EGF in cells to study the effects of EGF on cell proliferation, differentiation, and survival.

  • Generating stable cell lines expressing EGF for long-term studies of EGF signaling pathways and downstream effects.

  • Investigating the mechanisms of resistance to EGF inhibitors or combination therapies in cells that overexpress EGF.

  • Developing animal models of EGF-driven cancers by transfecting cells with pMSCV-EGF-GFP vectors and injecting them into animals.

  • Evaluating the efficacy of various inhibitors or combination therapies targeting EGF in vitro and in vivo.

pCMV-EGFR Vectors' Principle 

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death. Mutations in the EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.  

 

Downstream effects of EGFR activation include modulation of the RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

​

We offer a collection of unique cloning and expression cDNA vectors containing the wild-type or mutated EGFR gene. These mutations are clinically approved and are being studied by many researchers. Our high-quality vectors are fully sequenced and ready to use. We also offer custom cloning services for your specific construct needs.

EGFR mutations fig created 12-11-2017.jpg
pCMV-EGFR.png

Benefits

RT Research

Our engineered expression cell lines are perfect for studying RTKs and their pathways, or any other relevant molecular or relevant mechanisms.

Double Selection

Single clones are selected with GFP and hygromycin, which can be used to maintain RTK expression.

​Comprehensive Validation

The selected clones have been validated with PCR-sequencing and Western blot analysis.

Functional Analysis

Proliferation tests have been performed on the cell lines with their corresponding inhibitors.

pCMV-EGFR Vectors

Benefits

pLenti-MSCV-EGFR-GFP Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death.  Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.  Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

​

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.

pCMV-EGFR Vectors Research Application

​

  • Overexpressing EGFR in cells to study the effects of EGFR on cell proliferation, differentiation, and survival.

  • Generating stable cell lines expressing EGFR for long-term studies of EGFR signaling pathways and downstream effects.

  • Investigating the mechanisms of resistance to EGFR inhibitors or combination therapies in cells that overexpress EGFR.

  • Developing animal models of EGFR-driven cancers by transfecting cells with pCMV-EGFR vectors and injecting them into animals.

  • Evaluating the efficacy of various inhibitors or combination therapies targeting EGFR in vitro and in vivo.

pLenti-MSCV-EGFR-GFP Vectors

pLenti-MSCV-EGFR-GFP.png

Benefits

pLenti-MSCV-EGFR-GFP Vectors

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that plays a critical role in regulating cell division and death.  Mutations in EGFR gene that lead to overexpression of the protein have been associated with a number of different cancers. The EGFR gene is present on chromosome 7p11.2 and has 28 coding exons in which exons 5–7 and 13–16 code for the ligand-binding domain and exons 18–24 code for the TK domain.  Downstream effects of EGFR activation include modulation of three major pathways of RAS-RAF-MAPK, PI3K/AKT, and JAK/STAT pathways which can lead to cell survival, proliferation, and migration of cancer cells.

​

We offer a collection of unique cloning and expression cDNA vectors contain WT and mutated EGFR gene. These mutations are clinically approved and are under further investigation by many researchers. Our high-quality vectors are fully sequenced and are ready to use. We also offer custom cloning services for your specific construct needs.

pLenti-MSCV-EGFR-GFP Vectors Research Application

​

  • Generating stable cell lines expressing EGFR for long-term studies of EGFR signaling pathways and downstream effects.

  • Investigating the effects of EGFR mutations on downstream signaling pathways and cellular processes, such as cell proliferation, differentiation, and survival.

  • Identifying novel therapeutic strategies for targeting EGFR in cancer by testing the efficacy of various inhibitors or combination therapies.

  • Screening for small molecule compounds or antibodies that selectively target EGFR mutants over wild-type EGFR.

  • Developing animal models of EGFR-driven cancers by transfecting cells with pLenti-MSCV-EGFR-GFP vectors and injecting them into animals.

  • Evaluating the potential of EGFR and its mutants as diagnostic and prognostic biomarkers for specific types of cancer.

Benefits

Mutation
Product
SKU
Price (USD$)
WT

pCMV-EGFR (WT)

EV-0001
1529
C797S

pCMV-EGFR (C797S)

EV-0012
1529
T790M, C797S

pCMV-EGFR (T790M, C797S)

EV-0013
1911
L718Q, T790M, L858R

pCMV-EGFR (L718Q, T790M, L858R)

EV-0018
1911
H773insNPH

pCMV-EGFR (H773insNPH)

EV-0016
1911
T790M

pCMV-EGFR (T790M)

EV-0005
1529
Exon 19 Deletion

pCMV-EGFR Exon 19 Deletion

EV-0008
1529
L861Q

pCMV-EGFR (L861Q)

EV-0009
1529
G719A

pCMV-EGFR (G719A)

EV-0003
1529
Exon 20 Insertion

pCMV-EGFR Exon 20 Insertion

EV-0011
1529
D770_N771insSVD, C797

pCMV-EGFR (D770_N771insSVD, C797)

EV-0015
1911
G719C

pCMV-EGFR (G719C)

EV-0004
1529
L858Q

pCMV-EGFR (L858Q)

EV-0006
1529
Exon 19 Insertion

pCMV-EGFR Exon 19 Insertion

EV-0010
1529
L792H, L858R

pCMV-EGFR (L792H, L858R)

EV-0019
1911
T790M, L792H, L858R

pCMV-EGFR (T790M, L792H, L858R)

EV-0020
1911
L718Q, L858R

pCMV-EGFR (L718Q, L858R)

EV-0017
1911
L858R

pCMV-EGFR (L858R)

EV-0007
1529
T790M, C797S, Exon 19 Deletion

pCMV-EGFR (T790M, C797S, Exon 19 Deletion)

EV-0014
1911
G719S

pCMV-EGFR (G719S)

EV-0002
1529
WT

pLenti-EGFR (WT)

ELM-0001
1571
T790M, L792H, L858R

pLenti-EGFR (T790M, L792H, L858R)

ELM-0009
1935
A767_dupASV, C797S

pLenti-EGFR (A767_dupASV, C797S)

ELM-0002
1935
H773_V774insH, C797S

pLenti-EGFR (H773_V774insH, C797S)

ELM-0005
1935
Viii

pLenti-EGFR (Viii)

ELM-0010
1935
L792H, L858R

pLenti-EGFR (L792H, L858R)

ELM-0007
1935
L718Q, T790M, L858R

pLenti-EGFR (L718Q, T790M, L858R)

ELM-0008
1935
H773_V774iinsNPH

pLenti-EGFR (H773_V774iinsNPH)

ELM-0003
1935
D770_N771insSVD, C797S

pLenti-EGFR (D770_N771insSVD, C797S)

ELM-0004
1935
L718Q, L858R

pLenti-EGFR (L718Q, L858R)

ELM-0006
1935
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