TF Luciferase Reporter Vectors
Our TF Luciferase Reporter Vectors let you monitor individual transcription factor (TF) activity. Basic TF Firefly Luciferase vectors provide simple, reliable readouts, while Firefly–Renilla Dual vectors combine both reporters in a single vector, giving normalized measurements in one convenient system. Highly sensitive, ready-to-use substrates & lysis buffer are available for Firefly SKU# (LUCS100) & Firefly-Renilla Dual Luciferases SKU# (RLUCS050), optimized for use with these vectors. HygroSelect and NeoSelect vectors generate stable cell lines, with optional GFP in selectable vectors. We offer 100+ vectors in each category, all customizable for your research needs.
Principle
Each vector is designed to monitor the activity of a single transcription factor (TF), with at least four copies of a cis-acting enhancer element placed upstream of a minimal TATA promoter. Upon transfection and stimulation, activated TFs bind to the enhancer, triggering luciferase gene expression. The resulting chemiluminescent signal directly reflects TF activity.
Firefly–Renilla Dual vectors combine both reporters in a single construct to measure transcription factor activity with firefly luciferase while simultaneously normalizing for transfection efficiency using Renilla luciferase under a constitutive CMV promoter.
To generate stable cell lines, we offer over 50 HygroSelect and 50 NeoSelect vectors, each with hygromycin or neomycin resistance and GFP for visual selection. For dual-pathway monitoring in one cell line, use one vector with hygromycin and one with neomycin selection.
Signosis also provides custom vector services for unlisted TFs or selection markers. For more details or recommendations, contact us at 408-747-0771 or info@signosisinc.com.
For most high-throughput or relative-induction experiments, untreated wells with the pTA-TF vector are sufficient as a baseline, while a pTA-control vector is only needed for precise measurement of basal promoter activity.
Basic TF Firefly Luciferase Reporter Vectors
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To quickly monitor transcription factor activity in transiently transfected cells via firefly luciferase expression.
HygroSelect TF Firefly Luciferase Reporter Vectors with Optional GFP as a Selection Marker
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For generating stable cell lines to monitor transcription factor activity, with Hygromycin B selection and optional GFP visualization
NeoSelect TF Firefly Luciferase Reporter Vectors with GFP
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For generating stable cell lines to monitor transcription factor activity, with Neomycin selection and optional GFP visualization
Firefly–Renilla Dual Luciferase Reporter Vectors
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For measuring transcription factor activity with built-in normalization for accurate, fast results in transient assays
Product Categories
Benefits
All-in-One Vector
Both firefly (experimental) and Renilla (normalization) luciferases are included in a single construct, eliminating the need for separate plasmids or co-transfection.
Visualization
Some vectors include GFP, allowing fluorescence-based tracking of transfected cells.
Highly Sensitive Luciferase Bundles (Lysis & Substarte) for Detection
Highly Responsive
Incorporates an optimal consensus DNA binding sequence for enhanced transcription factor responsiveness and sensitivity.
Rapid, Reproducible Results
Enables sequential or multiplexed measurement of both reporters from the same sample for quick, sensitive, and consistent results in high-throughput and standard formats.
Customizable Solutions
Custom vector modifications and stable cell line services are available upon request.
Accurate Normalization
Renilla luciferase provides an internal standard, ensuring that firefly signal truly reflects changes in TF activity rather than experimental variability.
Flexible Experimental Design
Two sets of vectors enable stable expression of two different TFs in the same cell line.
