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TF Luciferase Vectors

Measure TF activation in cells. 100+ vectors are available. We also provide custom service to create a vector for any unlisted TFs.

 

pTF-Luc reporter vectors are a series of firefly luciferase-based reporter constructs for quantitative measurement of the activities of transcription factors (TFs) in cells. Each vector contains a cis-element (DNA binding sequence), a minimal promoter, and firefly luciferase gene. When a TF is activated, it binds to cis-element and results in induction of luciferase gene expression. Therefore, luciferase activity represents the activity of the TF.

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Principle

The Luciferase Reporter Vectors have been specially constructed to report the binding activity of a single TF. Multiple copies (at least four) of the cis-acting enhancer element have been inserted into each vector upstream of a minimal TA promoter. Upon vector transfection and cell stimulation, the activated TF is bound to the enhancer element and initiate the transcription of downstream luciferase reporter gene. The chemiluminescent measurement of luciferase activity is proportional to the amount of expressed enzyme and thus the binding activity of the targeted TF. 

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Transcription factor (TF) luciferase reporter vectors with hygromycin and/or GFP selection have been recently launched, containing a luciferase gene under the control of multiple repeats of TF consensus binding sequence and TATA minimal promoter, allowing researchers to monitor the activity of that promoter in different experimental conditions. In order to facilitate stable cell line generation, the vectors have been added selection markers, hygromycin resistance or GFP (Green Fluorescent Protein).  Hygromycin resistance allows for selection using the antibiotic hygromycin, while GFP provides a visible marker for identifying transfected cells.

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Benefits

Tailored Solutions for Any Pathway: Explore Customizable Single or Dual TF Luciferase Reporter Vectors

At Signosis, we offer a wide selection of TF Luciferase Reporter Vectors to meet diverse experimental needs, giving customers the flexibility to choose the most suitable option. Additionally, we provide custom services to create vectors for any unlisted transcription factors or selection markers.

To further enhance stable cell line generation, Signosis has introduced over 50+ TF Luciferase Reporter HygroSelect Vectors and 50+ TF Luciferase Reporter NeoSelect Vectors. These vectors feature either a hygromycin or neomycin resistance gene for effective selection, and also include GFP as a visible marker. For experiments requiring the monitoring of two distinct pathways in a single stable cell line, you can use two TF reporter vectors—one with neomycin selection and the other with hygromycin selection.

For more information or personalized recommendations, please contact us at 408-747-0771 or info@signosisinc.com.

Basic TF  Luciferase Reporter Vectors

Cost Effective solutions TF Reporter Vectors without any selection marker, suitable for transient transfection.

Can be used for creation of stable cell lines, but will require co-transfection with a resistance vector of your choice.

HygroSelect TF Luciferase Reporter Vectors

TF Reporter Vectors with the hygromycin resistance gene included.

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HygroSelect TF Luciferase Reporter Vectors with GFP

TF Reporter Vectors that include hygromycin resistance gene along with a GFP expression gene. The expression of GFP is suitable for both selection purposes and for confirming transfection efficiency.​

NeoSelect TF Luciferase Reporter Vectors 

TF Reporter Vectors with the neomycin resistance gene included.

NeoSelect TF Luciferase Reporter Vectors with GFP

TF Reporter Vectors that include neomycin resistance gene along with a GFP expression gene. The expression of GFP suitable for both selection purposes and for confirming transfection efficiency.​