miRNA Luciferase Reporter Vectors: Live-Cell Functional Analysis
Introduction
MicroRNAs, or miRNAs, are small non-coding RNA molecules that help regulate gene expression after transcription. Instead of completely turning genes on or off, miRNAs fine-tune gene activity by reducing protein translation or promoting mRNA degradation.
miRNA luciferase reporter assays are commonly used to confirm whether a miRNA directly binds to a target gene’s 3' UTR. In this system, the target 3' UTR is placed downstream of a luciferase reporter. When the miRNA binds to the target sequence, it suppresses luciferase expression, causing the light signal to decrease. Therefore, stronger miRNA activity results in lower luminescence activity.
This inverse relationship makes miRNA luciferase reporters a useful tool for measuring miRNA function, validating miRNA-mRNA interactions, and studying post-transcriptional gene regulation. Because abnormal miRNA activity is linked to cancer progression, tumor development, and therapeutic resistance, these assays are also valuable for cancer biomarker research.
Signosis offers over 100 validated miRNA Luciferase Reporter Vectors for quantitative live-cell analysis of miRNA activity and target suppression. For miRNA targets not available in our standard catalog, Signosis also provides custom 3' UTR vector construction services.
Product Specifications
Reporter System: Firefly Luciferase Bioluminescent Readout
Target Mechanism: Direct 3' UTR Complementary Binding
Library Size: Over 100 Pre-Validated Target Vectors In Stock
Applications: Quantitative miRNA Profiling, Cancer Biomarker Discovery, Live-Cell Assays
Custom Services: Custom specific miRNA and 3' UTR Vector Construction & Sequence Modifications
Assay Principle
Signosis miRNA reporter plasmids utilize a highly sensitive chemiluminescent readout to measure functional microRNA activity in mammalian cells.
1. Optimized Vector Architecture
Each reporter vector features a specialized design: a fully complementary microRNA (miRNA) target sequence is engineered directly into the 3' UTR, immediately downstream of the firefly luciferase reporter gene.
2. Targeted Translation Suppression
Following transient transfection into mammalian cells, endogenous or transfected miRNAs bind to their exact match in the 3' UTR. This specific binding recruits the RNA-induced silencing complex (RISC), suppressing luciferase expression.
3. High-Sensitivity Bioluminescent Readout
The remaining functional luciferase enzyme processes its substrate to generate a stable light signal. Because RISC binding represses translation, luminescent intensity is inversely proportional to active miRNA concentration. This allows for precise, high-throughput quantification of miRNA activity shifts across experimental or drug screening groups.
Benefits
Direct Intracellular Readout
Quantify actual functional miRNA activity inside live cells rather than static cell lysates
High-Sensitivity Bioluminescence
Features a wide dynamic range with exceptional signal-to-noise ratios compared to traditional Northern blotting
Extensive Target Selection
Off-the-shelf coverage for major human, mouse, and rat microRNA pathways
Seamless Customization
Rapid turnaround on custom cloning designs for novel or mutated miRNA target sequences.
Products
Target | Product | SKU | Price (USD$) |
|---|---|---|---|
Let7b | LR-0001 | 415 | |
MiR-7-5p | LR-0002 | 415 | |
MiR-7b-5p | LR-0003 | 415 | |
MiR-15a-5p | LR-0004 | 415 | |
MiR-16-5p | LR-0005 | 415 | |
MiR-17-5p | LR-0006 | 415 | |
MiR-17-3p | LR-0007 | 415 | |
MiR-19a-3p | LR-0008 | 415 | |
MiR-20a-5p | LR-0009 | 415 | |
MiR-21-5p | LR-0010 | 415 | |
MiR-24-3p | LR-0011 | 415 | |
MiR-26a-5p | LR-0012 | 415 | |
MiR-29a-3p | LR-0013 | 415 | |
MiR-34a-5p | LR-0014 | 415 | |
MiR-101-3p | LR-0015 | 415 | |
MiR-106a-5p | LR-0016 | 415 | |
MiR-107 | LR-0017 | 415 | |
MiR-122a-5p | LR-0018 | 415 | |
MiR-125a-5p | LR-0019 | 415 | |
MiR-125b-5p | LR-0020 | 415 | |
MiR-127-5p | LR-0021 | 415 | |
MiR-132-3p | LR-0022 | 415 | |
MiR-144-3p | LR-0023 | 415 | |
MiR-146a-5p | LR-0024 | 415 | |
MiR-155-5p | LR-0025 | 415 | |
MiR-181a-5p | LR-0026 | 415 | |
MiR-194-5p | LR-0027 | 415 | |
MiR-196a-5p | LR-0028 | 415 | |
MiR-199a-5p | LR-0029 | 415 | |
MiR-206 | LR-0030 | 415 | |
MiR-214-3p | LR-0031 | 415 | |
MiR-221-3p | LR-0032 | 415 | |
MiR-222-3p | LR-0033 | 415 | |
MiR-372-3p | LR-0035 | 415 | |
MiR-373-5p | LR-0036 | 415 | |
Let7a | LR-0037 | 415 | |
Let7c | LR-0038 | 415 | |
Let7d | LR-0039 | 415 | |
Let7e | LR-0040 | 415 | |
Let7f | LR-0041 | 415 | |
Let7g | LR-0042 | 415 | |
Let7i | LR-0043 | 415 | |
MiR-1 | LR-0044 | 415 | |
MiR-10a-5p | LR-0045 | 415 | |
MiR-10b-5p | LR-0046 | 415 | |
MiR-143-3p | LR-0047 | 415 | |
MiR-145-5p | LR-0048 | 415 | |
MiR-15b-5p | LR-0049 | 415 | |
MiR-181b-5p | LR-0050 | 415 | |
MiR-18a-5p | LR-0051 | 415 | |
MiR-19b-3p | LR-0053 | 415 | |
MiR-9-5p | LR-0054 | 415 | |
MiR-205-5p | LR-0055 | 415 | |
MiR-208a-3p | LR-0056 | 415 | |
MiR-34b-5p | LR-0057 | 415 | |
MiR-34c-5p | LR-0058 | 415 | |
MiR-210-3p | LR-0059 | 415 | |
MiR-451a | LR-0060 | 415 | |
MiR-106b-3p | LR-0061 | 415 | |
MiR-18b-5p | LR-0062 | 415 | |
MiR-29b-3p | LR-0063 | 415 | |
MiR-153-3p | LR-0064 | 415 | |
MiR-223-3p | LR-0066 | 415 | |
MiR-380-5p | LR-0067 | 415 | |
MiR-496a-3p | LR-0068 | 415 | |
MiR-448 | LR-0069 | 415 | |
MiR-885-5p | LR-0070 | 415 | |
MiR-455-5p | LR-0071 | 415 | |
MiR-208b-3p | LR-0072 | 415 | |
MiR-200c-3p | LR-0073 | 415 | |
MiR-29c-3p | LR-0074 | 415 | |
MiR-28 | LR-0075 | 415 | |
MiR-122 | LR-0076 | 415 | |
MiR-141 | LR-0077 | 415 | |
MiR-198 | LR-0078 | 415 | |
MiR-200 | LR-0079 | 415 | |
MiR-320 | LR-0080 | 415 | |
MiR-349 | LR-0081 | 415 | |
MiR-451 | LR-0082 | 415 | |
MiR-655 | LR-0083 | 415 | |
MiR-181 | LR-0084 | 415 | |
MiR-101a | LR-0085 | 415 | |
MiR-200a | LR-0086 | 415 | |
MiR-19-5p | LR-0087 | 415 | |
MiR-27a | LR-0088 | 415 | |
MiR-30b | LR-0089 | 415 | |
MiR-380 | LR-0090 | 415 | |
MiR-885 | LR-0091 | 415 | |
MiR-99a | LR-0092 | 415 | |
MiR Control | LR-1000 | 415 |
