miRNA Luciferase Reporter Vectors
miRNAs have been shown to regulate many developmental and physiological processes, and the deregulation of miRNAs has been linked to a number of disease processes, including cancer. miRNAs play important roles in fine-tuning gene expression regulators referred to as “dimmer switches” because of their ability to repress gene expression without completely silencing it. The precise monitoring of miRNA expression particularly in cell-based assay, can help to reveal the potential miRNA impacts on cancer etiology, differential stages and therapy development. Signosis developed the miRNA reporter vectors. Each reporter vector was constructed by inserting miRNA exactly complementary sequence (~20nt) as corresponding miRNA binding sites on the downstream of luciferase reporter gene. When miRNA in cells binds to the complementary site at 3'UTR downstream of reporter luciferase gene, it leads to repression of the expression of luciferase and results in reduction of luciferase enzyme activity. The miRNA expression level quantitatively corresponds with the luciferase activities.
100+ luciferase reporter vectors are available for monitoring different miRNAs in vivo. Signosis also provides custom services for any unlisted miRNA that you might be interested in.
The specific miRNA complementary sequence has been engineered at 3' untranslated region of luciferase gene to make a miRNA luciferase reporter vector. When miRNA targets the binding site at 3'UTR downstream of reporter luciferase gene, it leads to repression of the expression of luciferase and results in reduction of luciferase enzyme activity. Through comparison of luciferase activity in two samples, up- or down-regulated miRNA can be quantitatively measured.