RBP-16 RNA EMSA Array
Parallel Profiling of 16 Post-Transcriptional Regulators
The Signosis RBP-16 RNA EMSA Array allows you to simultaneously profile the activity of 16 key RNA-binding proteins (RBPs) in a single experiment. Fully validated for human, mouse, and rat samples, this plate-based assay replaces tedious individual gel-shift assays with a fast, scalable workflow. By screening multiple pathways at once, you can efficiently monitor dynamic shifts in RBP activity across different drug treatments, disease models, or genetic modifications.
Principle
The array eliminates traditional gel electrophoresis by pairing liquid-phase binding with plate hybridization:
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Binding: Cell or tissue extracts are incubated with a mixture of 16 distinct, biotin-labeled RNA probes. Active RBPs bind to their corresponding probes.
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Separation: The mixture is passed through a spin column. Intact RBP/RNA complexes are retained, while unbound probes are washed away.
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Hybridization: The complexes are denatured to release the biotinylated RNA probes, which are then hybridized to a 96-well plate pre-coated with complementary target sequences.
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Detection: Bound probes are quantified using a streptavidin-HRP conjugate and an ultra-sensitive chemiluminescent substrate on a standard plate reader.
Covered RNA-Binding Proteins (16 Targets)
HuR (ELAVL1) | PTBP1 | LIN28A | SLBP |
TTP (ZFP36) | TDP-43 (TARDBP) | Pumilio (PUM1/2) | HCV IRES–eIF3 |
AUF1 (HNRNPD) | SRSF1 (ASF/SF2) | IRP1 | Smaug |
CELF1 (CUGBP1) |
NOVA1 / NOVA2 | IRP2 |
Staufen |
Benefits
Targeted
Profiling
Profile 16 critical RNA-binding motifs in parallel, enabling comprehensive pathway analysis while saving time and sample volume.
Ready-to-Use & Validated System
Includes pre-validated RNA probes, optimized buffers, spin columns, and controls, minimizing assay development and ensuring reproducibility.
Replaces Tedious Gel Shifts (EMSAs)
Transforms traditional gel shift assays into a fast, plate-based format, eliminating gels, simplifying workflow, and increasing throughput.
Physiologically Relevant Readouts
Measures endogenous RBP-binding activity directly from cell or tissue lysates, preserving native binding interactions.
Quantitative & Sensitive Readout
Biotin-chemiluminescent detection enables sensitive, quantitative measurement of RNA–protein binding activity.
Broad Coverage of RNA Regulation
Captures major post-transcriptional mechanisms, including mRNA stability, splicing, translation, and miRNA maturation.
High-Throughput Screen Ready
96-well plate format enables parallel analysis of multiple samples, treatments, or compounds.
Safe, Accessible & Compatible
Non-radioactive, plate reader–based workflow requires no specialized infrastructure and is validated for human, mouse, and rat samples.