Signosis' EL-039 is a stable Ba/F3 cell line expressing EGFR with the H773_V774insH insertion in the αC helix loop and the C797S mutation. The cell line was generated by electroporation with a vector containing the mutant EGFR gene, along with GFP and hygromycin resistance. Clones were selected based on hygromycin resistance, GFP expression, and confirmed mutant EGFR protein expression.
EL-039 EGFR (H773_V774insH, C797S) BaF3 Stable Cell Line
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