Signosis' EL-0036 is a stable Ba/F3 cell line expressing EGFR with the viii mutation, a gain-of-function deletion of exons 2–7. The cell line was generated by electroporation with a vector containing the mutant EGFR gene, along with GFP and hygromycin resistance. Clones were selected based on hygromycin resistance, GFP expression, and confirmed EGFRvIII protein expression.
EL-036 EGFR (viii) BaF3 Stable Cell Line
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