E2F is a family of transcription factors that plays a crucial role in regulating the cell cycle, DNA replication, and cellular response to growth signals. The E2F luciferase reporter vector is designed to study the transcriptional activity of E2F. The vector contains an E2F DNA consensus binding site and TA minimal promoter upstream of a firefly luciferase reporter gene, which can specifically report E2F activity while maintaining a low basal level of transcription. It also contains the Renilla Luciferase under the CMV promoter for data normalization.