The erythroblast transformation specific (ETS) family of transcription factors is involved in the regulation of numerous cellular processes including hematopoiesis, vascular development, immune responses, oncogenesis, and neurogenesis. The ETS luciferase reporter vector is designed to study the transcriptional activity of ETS. The vector contains an ETS DNA consensus binding site and TA minimal promoter upstream of a firefly luciferase reporter gene, which can specifically report ETS activity while maintaining a low basal level of transcription. It also contains the Renilla Luciferase under the CMV promoter for data normalization.