SP1 is a member of the SP/Kruppel-like factor family of transcription factors and is involved in the regulation of many housekeeping genes, as well as genes involved in cell growth, differentiation, and response to external signals. The SP1 luciferase reporter vector is designed to study the transcriptional activity of SP1. The vector contains an SP1 DNA consensus binding site and TA minimal promoter upstream of a firefly luciferase reporter gene, which can specifically report SP1 activity while maintaining a low basal level of transcription. It also contains the Renilla Luciferase under the CMV promoter for data normalization.