Lentiviral Expression System
Introduction
Lentiviral gene delivery is one of the most powerful tools in modern molecular biology—but self-optimized or unstandardized production workflows are often complex, time-consuming, and highly variable. Viral yield and performance can vary significantly depending on producer cell condition, precise packaging vector ratios, and transfection efficiency.
To eliminate these inconsistencies and deliver reliable, high-titer results, Signosis has developed a standardized, high-performance lentiviral production system designed for simplicity, reproducibility, and efficiency.
Signosis Lentiviral Production System (SKU: LV-0001) provides everything you need for consistent, high-quality lentiviral production. The All-in-One includes:
-
LentiPack HEK293T producer cell line (SKU: PC-023)
-
PEI transfection reagent (SKU: CC-0011)
-
lentiviral packaging mix (SKU: ELM-016)
-
Polybrene infection reagent (SKU: CC-0012)
This cost-effective, integrated solution is ideal for beginners as well as routine laboratory research and high-throughput applications, delivering reliable performance and reproducible results.
A wide range of ready-to-use lentiviral expression vectors are available for efficient gene delivery and stable gene expression in mammalian cells. These vectors are fully compatible with the Signosis Lentiviral Production System (SKU: LV-0001), enabling the generation of high-titer, replication-deficient lentiviral particles.
In addition, Signosis offers custom vector services, including flexible reporter options such as GFP and selectable markers, to support your specific research and therapeutic development needs.
Principle
Lentiviral expression relies on the natural ability of lentiviruses to deliver genetic material into host cells and integrate it into the genome. In a typical system:
-
Clone the gene of interest into a lentiviral expression vector containing appropriate regulatory elements.
-
Co-transfect the expression vector into LentiPack HEK293T cells (SKU: PC-023) along with helper plasmids (SKU: ELM-016) using the PEI Transfection Reagent (SKU: CC-0011).
-
Lentiviral particles are efficiently assembled and released into the culture medium.
-
Transduce target cells with the viral particles using the Polybrene Infection/Transduction Reagent (SKU: CC-0012).
-
The inserted gene is stably integrated and expressed, making the system suitable for downstream applications.
This system combines high efficiency, broad cell-type compatibility, and long-term stability while maintaining safety and reproducibility in laboratory use.
Key benefits
-
User‑friendly — ideal for both new users and experienced researchers.
-
All‑in‑one convenience — no more sourcing components from multiple vendors.
-
High, consistent viral titers powered by optimized HEK293T producer cells.
-
Reproducible transfection using validated PEI and packaging mix.
-
Works with difficult cell types, including suspension cells, primary cells, and hard‑to‑transfect lines.
-
Flexible custom vector options, including GFP, selectable markers, and reporter constructs.
Lentiviral Production Components
Name | SKU | Formatted Price |
|---|---|---|
PEI transfection reagent | CC-0011 | $60.00 |
Polybrene Infection / Transduction Reagent | CC-0012 | $69.00 |
pLP1 vector | ELM-0013 | $99.00 |
pLP2 vector | ELM-0014 | $99.00 |
pLP/VSVG vector | ELM-0015 | $99.00 |
Lentiviral Packaging Mix (25 reactions) | ELM-0016 | $399.00 |
Lentiviral Production System | LV-0001 | $880.00 |
LentiPack 293T | PC-023 | $445.00 |
Lentiviral Transfer Vectors
Name | SKU | Formatted Price |
|---|---|---|
pLenti-EGFR (A767_dupASV, C797S) | ELM-0002 | $1,935.00 |
pLenti-EGFR (D770_N771insSVD, C797S) | ELM-0004 | $1,935.00 |
pLenti-EGFR (H773_V774iinsNPH) | ELM-0003 | $1,935.00 |
pLenti-EGFR (H773_V774insH, C797S) | ELM-0005 | $1,935.00 |
pLenti-EGFR (L718Q, L858R) | ELM-0006 | $1,935.00 |
pLenti-EGFR (L718Q, T790M, L858R) | ELM-0008 | $1,935.00 |
pLenti-EGFR (L792H, L858R) | ELM-0007 | $1,935.00 |
pLenti-EGFR (T790M, L792H, L858R) | ELM-0009 | $1,935.00 |
pLenti-EGFR (Viii) | ELM-0010 | $1,935.00 |
pLenti-EGFR (WT) | ELM-0001 | $1,571.00 |
pLenti-MSCV-EGFR-GFP-HYG Vector, EGFR (Del19), non-FLAG tagged | ELM-0011 | $1,935.00 |
pLenti-MSCV-EGFR-GFP-HYG Vector, EGFR (L858R), non-FLAG tagged | ELM-0012 | $1,935.00 |