Glucocorticoid Receptor/GR
Principle
The Glucocorticoid receptor (GR) is a major player in development, metabolism and immune response. When activated by stimuli, GR translocates from the cytoplasm into the nucleus, and then binds a DNA recognition site to regulate gene expression. In addition, activated GR can transrepress other transcription factors that have been misregulated in cancer and other diseases.
The cell line was established by transfection of a GR firefly luciferase reporter vector along with antibiotic (Hygromycin or G418) resistance expression vector for selection. The antibiotic resistant clones were subsequently screened for DEX-induced luciferase activity. The clone with the highest fold induction was selected and expanded to produce this stable cell line.
Benefits
High Sensitivity & Responsiveness
Each cell line is validated to induce a strong reporter signal in response to stimuli.
Consistent
TF reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
Time-Saving
Cell line can be used for experiments right away to study different signaling pathways.
Routine Mycoplasma Testing
All cell lines tested negative for mycoplasma.
