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Mitochondria Activity Assay Kit

The mitochondria, which is known as the powerhouse of the cell, generates chemical energy for the cell in the form of ATP. ATP is produced by the mitochondria through aerobic respiration, a catabolic process that utilizes the mitochondria’s membrane potential to convert ADP to ATP. Since the mitochondria’s membrane potential is correlated to how active it is, the activity levels of mitochondria can be determined by measuring its membrane potential. Signosis has developed a kit that utilizes the TMRE dye to detect mitochondria activity.

Principle

The Mitochondria Activity Assay kit utilizes tetramethylrhodamine ethyl ester (TMRE), a cell permeable red-orange dye to measure differences in mitochondria membrane potential. Since TMRE is positively charged, it accumulates in active mitochondria, which has a negative charge. In contrast, inactive mitochondria will have less TMRE binding and a lower signal due to its decreased membrane potential. 

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