top of page

Mitochondria Activity Assay Kit

Signosis’ Mitochondrial Activity Detection Kit offers a reliable method for evaluating mitochondrial function by measuring membrane potential. Using TMRE dye, this assay enables researchers to monitor mitochondrial health and activity levels in live cells—ideal for studies involving metabolism, drug response, apoptosis, and mitochondrial disorders.

Principle 

The Mitochondria Activity Assay kit utilizes tetramethylrhodamine ethyl ester (TMRE), a cell permeable red-orange dye to measure mitochondria activity by detecting differences in mitochondria membrane potential. Since TMRE is positively charged, it accumulates in active mitochondria, which has a negative charge. In contrast, inactive mitochondria will have less TMRE binding and a lower signal due to its decreased membrane potential. 

Mitochondria Activity Assay Kit (1).png

Benefits

Includes Negative Control

Ensures assay reliability by confirming background activity levels.

Measures Mitochondrial Function

MRE dye detects membrane potential, reflecting mitochondrial activity.

Compatible with Live Cells

Provides an accurate measure of mitochondrial activity in live cells without disrupting their integrity.

​Versatile for Multiple Applications

​Ideal for studies on apoptosis, metabolism, drug response, and mitochondrial diseases.

Products

bottom of page