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Human Autoimmune ELISA Kits

Signosis offers ELISA kits designed for the sensitive detection of autoimmune antibodies in human samples. Featuring a convenient 12×8-well plate with removable strips, these kits provide flexibility, ease of use, and reliable results for studying autoimmune responses in human models.

Principle 

​Signosis’ Autoimmune ELISA Kits detect autoimmune antibodies in human serum using a standard ELISA format. A specific antigen is pre-coated onto microtiter wells to capture the target antibodies. During the assay, the sample is added, and autoimmune antibodies, if present, bind to the immobilized antigen. An HRP-conjugated anti-human IgG antibody is then added, forming a complex. After washing away unbound proteins, TMB substrate is added, which reacts with HRP to form a blue color. Then, Stop solution is added to terminate the reaction and change the blue color to yellow. The intensity of the yellow color, measured at an absorbance of 450 nm, is directly proportional to the concentration of autoimmune antibodies in the sample.

Autoimmune ELISA diagram.gif

Benefits

User-Friendly Design

Pre-coated plates with 12x8 detachable strips provides flexibility in experiments.

Compatible with Multiple Sample Types

Suitable for serum, plasma, and other sample types from human subjects.

Comprehensive Selection of Markers

Analyze a wide range of autoimmune antibodies, including antibodies which target dsDNA, SSA/Ro, SSB/La, histone, and more.

Antigen
Product
SKU
Price (USD$)
8 Antigen Panel
EA-5101
713
dsDNA
EA-5002
570
SSA (Ro-60)
EA-5003
570
SSA (Ro-52)
EA-5004
570
SSB (La)
EA-5005
570
Scl-70
EA-5007
570
ANA
EA-5013
570
CENP-B
EA-5008
570
Jo-1
EA-5009
570
SmD1
EA-5011
570
Histone
EA-5010
570
U1-snRNP (68 kDa)
EA-5006
570
GAD65
EA-5015
570

Product

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